Melatonin benefits to the growth of human annulus fibrosus cells through inhibiting miR-106a-5p/ATG7 signaling pathway

Abstract

Background

Disc degeneration (DD) is one of the common diseases worldwide, which deeply influences normal life and leads to excruciating pain. However, an effective treatment for DD is still not identified.

Method

The present study systemically examined the effect of melatonin on annulus fibrosus (AF) cells of patients with DD.

Results

Melatonin had the effect of promoting proliferation, inducing autophagy, and suppressing apoptosis on AF cells of patients with DD. Moreover, melatonin contributed to the translation and transcription of autophagy-related protein ATG7 and inhibited the function of miR-106a-5p in AF cells. In addition, the results suggested that miR-106a-5p mediated the expression of ATG7 by directly binding to its 3′UTR in AF cells.

Conclusion

This research not only gained a deep insight of melatonin mode of action, but also indicated its potential target signaling pathway in AF cells.

Keywords:

• disc degeneration
• melatonin
• ATG7
• miR-106a-5p

Acknowledgments

This study was supported by the fund for Forstering Young Scholar of Peking University Health Science Center (NO BMU2017PY017).

Disclosure

The authors report no conflicts of interest in this work.