Hsa-miR-149-5p Suppresses Prostate Carcinoma Malignancy by Suppressing RGS17

Abstract

Background

MicroRNAs (miRNAs) are key players in the progression of human cancers. While several miRNAs have been reported to regulate the development of tumors, the molecular mechanisms and roles of miR-149-5p in prostate carcinoma (PCa) remain unclear. Our aim was to investigate the interaction and functions of miR-149-5p and RGS17 in PCa.

Methods

Microarray analysis was performed to identify the key miRNA and gene involved in PCa progression. The expression levels of miRNA and mRNA in PCa tissues and cells were verified by qRT-PCR. MTT assay, BrdU proliferation assay and wound-healing assay were applied to assess the effect of miR-149-5p and RGS17 on PCa cells’ viability, proliferation, and migration ability. The association between RGS17 and miR-149-5p was identify using dual-luciferase reporter assay and Western blot assay.

Results

Data analysis indicated the reduction of miR-149-5p expression in PCa tissues and cells. Experimental investigations also showed that this miRNA suppressed the viability, proliferation and migration ability of PCa cells. RGS17 was found to be the target of miR-149-5p, and the low expression of miR-149-5p upregulated RGS17 in PCa tissues and cells. The results of the cell-function assays showed that RGS17 acted as an oncogene in PCa even though its promotive effect could be reversed by miR-149-5p.

Conclusion

This research confirmed that by targeting and inhibiting RGS17, miR-149-5p could suppress PCa development.

Keywords:

• miR-149-5p
• prostate carcinoma
• PCa
• RGS17
• malignancy

Abbreviations

PCa, Prostate carcinoma; miRNA, MicroRNA; UTR, untranslated region ; RGS17, The Regulator of G Protein Signaling 17; GAP, GTPase activating protein; DEGs, differentially expressed genes; NC, negative control.

Data Sharing Statement

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Ethics Approval

The present study was approved by the Ethics Committee of the Third People’s Hospital of Hubei Province (Wuhan, China).

Consent for Publication

Consent for publication was obtained from the participants.

Disclosure

The authors declare that they have no conflict of interests for this work.