Clinical Significance of miR-183-3p and miR-182-5p in NSCLC and Their Correlation

Abstract

Purpose

Accumulating evidence has indicated that dysregulated microRNAs (miRNAs) are involved in cancer progression. In this study, we evaluated the clinicopathologic significance of miR-183-3p and miR-182-5p, and the role of miR-183-3p in non-small-cell lung cancer (NSCLC) progression.

Patients and Methods

Seventy-six NSCLC patients from Beijing Chest Hospital were included. The expression of miR-183-3p and miR-182-5p was evaluated by real-time quantitative polymerase chain reaction (RT-qPCR). Then, cell growth curve assays and colony formation assays were performed. Bioinformatics analysis of TCGA database was performed to explore the clinicopathological significance and prognostic value.

Results

miR-183-3p and miR-182-5p were significantly increased in NSCLC tumor tissues (both P < 0.0001) and were positively correlated (r = 0.8519, P < 0.0001). miR-183-3p (P = 0.0444) and miR-182-5p (P = 0.0132) were correlated with tumor size. In addition, miR-183-3p (P = 0.0135) and miR-182-5p (P = 0.0009) were upregulated in normal lung tissues from smokers. In vitro, miR-183-3p was correlated with cell proliferation. In addition, bioinformatics analysis indicated that miR-183-3p was correlated with poor prognosis (P = 0.0466) and tumor size (P = 0.0017). In addition, miR-183-3p was higher in lung squamous carcinoma (LUSC) tissue (P < 0.0001) than in lung adenocarcinoma (LUAD) tissue, and miR-183-3p was higher in the tumor tissue of smokers (P = 0.0053) than in that of nonsmokers.

Conclusion

Upregulation of miR-183-3p and miR-182-5p may play an oncogenic role in NSCLC. miR-183-3p could be used as a potential prognostic biomarker and therapeutic target to manage lung cancer.

Keywords:

• miR-183-3p
• miR-182-5p
• non-small-cell lung cancer
• NSCLC
• proliferation
• prognosis

Data Sharing Statement

The datasets used during the current study are available from the corresponding authors on reasonable request.

Ethical Approval and Exemption of Informed Consent

All specimens in this study were collected from the Clinical Resources Bio-Bank of Beijing Chest Hospital, which was approved by the Ethics Committee of the Beijing Chest Hospital, Capital Medical University. The resource collection and utilization plan of the Bio-Bank was approved by the Ethics Committee of Beijing Chest Hospital, Capital Medical University. The samples were obtained from the surgical resection tissue after pathological examination in Beijing Chest Hospital, Capital Medical University. This study started after patients were discharged from the hospital, and it was impossible to obtain informed consent. Hence, the Ethics Committee approved exemption of informed consent. This study was strictly in accordance with the ethical standards of the Ethics Committee of Beijing Chest Hospital, Capital Medical University and with the Helsinki Declaration and its later amendments. This study strictly protects patients’ privacy and personal information confidentiality. Ethical Approval Number: 2019-71.

Consent for Publication

The information disclosed in this study does not involve identifiable information for included individuals.

Disclosure

The authors have no conflicts of interest to declare that are relevant to the content of this article.