https://orcid.org/0000-0001-6626-4939
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Abstract
Purpose
The role of the peroxiredoxin (PRDX) family in oral squamous cell carcinoma (OSCC) remains unclear. This study aimed to investigate the expression of PRDXs and their effects on the prognosis in OSCC.
Methods
The expression of PRDXs and their effects on prognosis were analysed in 216 OSCC samples from The Cancer Genome Atlas (TCGA) database. OSCC tissues and adjacent noncancerous tissues (ANTs) were obtained from 68 clinical patients. Quantitative real-time (qRT)-PCR, Western blot, and immunohistochemical (IHC) staining were used to verify the relationship between the expression level of PRDX1 and different clinical features. Gene set enrichment analysis (GSEA) was used to examine the molecular mechanism of PRDX1 in OSCC.
Results
PRDX1 was found to be the only gene in PRDX family that highly expressed in OSCC samples and affected the prognosis of patients with OSCC. PRDX1 expression was significantly related to tumor stage, lymphatic metastasis, and pathological grade. A nomogram consisting of tumor stage, N stage, and PRDX1 level was constructed. GSEA showed that high expression of PRDX1 involved many cancer-related molecular functions and signaling pathways.
Conclusion
PRDX1 may play an important role in the occurrence and development of OSCC, and may be a potential new target for OSCC treatment.
Acknowledgments
This study was supported by the Beijing Municipal Natural Science Foundation of China (Grant No: 7192075).
Abbreviations
HNC, head and neck cancer; OSCC, oral squamous cell carcinoma; ROS, reactive oxygen species; PRDXs, peroxiredoxins; Trx, thioredoxin; TCGA, The Cancer Genome Atlas; ANTs, adjacent noncancerous tissues; TPM, transcripts per million; KM, Kaplan-Meier; RIPA, radioimmunoprecipitation assay; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; PVDF, polyvinylidene difluoride; qRT, quantitative reverse transcription; IHC, Immunohistochemical; HRP, horseradish peroxidase; MOD, mean optical density; GSEA, gene set enrichment analysis; GO, gene ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; AUC, area under the curve; Cys, cysteine.
Data Sharing Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Ethical Approval Statement
This research complied with the Declaration of Helsinki and was approved by the Research Ethics Committee of the Beijing Stomatological Hospital of Capital Medical University (Approval No. CMUSH-IRB-KJ-PJ-2018-01). All patients agreed to use their samples in this study and signed written informed consent.
Author Contributions
All authors contributed to data analysis, drafting or revising the article, have agreed on the journal to which the article will be submitted, gave final approval of the version to be published, and agree to be accountable for all aspects of the work.
Disclosure
The authors declare that there is no conflict of interest that can affect the publication of this article.