Aberration of lncRNA LINC00460 is a Promising Prognosis Factor and Associated with Progression of Clear Cell Renal Cell Carcinoma

Abstract

Purpose

Long noncoding RNAs have been studied more and more as potential prognostic markers. However, the prognostic of LINC00460 in clear cell renal cell carcinoma (ccRCC) has not been explored. In this study, the potential role of LINC00460 was investigated in ccRCC.

Patients and Methods

One hundred thirteen pairs of ccRCC tissues and para-normal tissues were collected. The expressions of LINC00460 in these tissues and ccRCC cells were evaluated via qRT-PCR. The prognostic value of LINC00460 was accessed with the use of Kaplan–Meier analysis and Cox proportional hazards model analysis. The influence of LINC00460 on ccRCC cell proliferation, migration, and invasion was determined via cell counting kit-8 (CCK-8) and Transwell assays.

Results

The results revealed that LINC00460 was significantly enhanced in ccRCC tissues, as well as in ccRCC cell lines. The overexpression of LINC00460 was significantly associated with lymph node metastasis and TNM stage, and lead to poor overall survival. Knockdown of LINC00460 reduces the cell ability of proliferation, migration, and invasion. LINC00460 could sponge to miR-149-5p.

Conclusion

LINC00460 may be developed as a prognostic biomarker and molecular therapy target for ccRCC.

Keywords:

• LINC00460
• clear cell renal cell carcinoma
• miR-149-5p
• prognosis

Ethics Statement

All participants gave their written informed consent when enrolled. The study was approved by the Ethics Committee of The Second Affiliated Hospital of Mudanjiang Medical University and also conducted following the Declaration of Helsinki.

Disclosure

The authors report no conflicts of interest in this work.