Prognostic Significance of Circulating Lymphocyte Subsets Before Treatment in Patients with Nasopharyngeal Carcinoma

Abstract

Purpose

We set out to explore the prognostic value of circulating lymphocyte subsets in patients with nasopharyngeal carcinoma (NPC) before treatment and to investigate changes in lymphocyte subsets resulting from chemoradiotherapy.

Patients and Methods

This retrospective study included 677 patients with non-metastatic NPC. The cutoff value of lymphocyte subsets was determined by the receiver operating characteristic curve (ROC), and the prognostic significance of lymphocyte subsets was evaluated by the Log rank test and Cox proportional hazards model. The endpoints were overall survival (OS), progression-free survival (PFS), locoregional relapse-free survival (LRFS) and distant metastasis-free survival (DMFS). Differences in lymphocyte subsets before and after chemoradiotherapy were analyzed by Wilcoxon signed rank test.

Results

NPC patients with high levels of CD19⁺ B cells (>9.55%) had better 5-year OS (90.4% VS 76.8%, P < 0.001), 5-year PFS (85.3% VS 71.6%, P < 0.001) and 5-year DMFS (94% VS 86.8%, P = 0.002) than patients with low levels of CD19⁺ B cells. Patients with high levels of CD4⁺ T cells (> 37.05%) had better 5-year PFS (83% VS 74.2%, P = 0.015) and better 5-year DMFS (95.8% VS 86.7%, P < 0.001) than those with low levels of CD4⁺ T cells. Multivariate analyses indicated that CD19⁺ B cell was an independent prognostic factor for OS, PFS and DMFS in NPC. And CD4⁺ T cell was an independent prognostic factor for PFS and DMFS. Within 1 month after chemoradiotherapy, the percentages of CD4⁺ T cells, CD19⁺ B cells, and the CD4/CD8 ratio decreased significantly, while the percentages of CD8⁺ T cells increased significantly.

Conclusion

NPC patients with low levels of CD19⁺ B cells or CD4⁺ T cells before treatment have a poor prognosis. In addition, chemoradiotherapy may reduce the body’s immune function in NPC patients.

Keywords:

• nasopharyngeal carcinoma
• lymphocyte subsets
• prognosis
• chemoradiotherapy
• immune function

Abbreviations

NPC, Nasopharyngeal carcinoma; ROC, Receiver operating characteristic; OS, Overall survival; PFS, Progression-free survival; LRFS, Locoregional relapse-free survival; DMFS, Distant metastasis-free survival; KPS, Karnofsky Performance Status; IMRT, Intensity modulated radiotherapy; AJCC, American Joint Committee on Cancer; GTV, Gross tumor volume; PTV, Planning target volume; CTV, Clinical target volume; NLR, Neutrophil-to-lymphocyte ratio.

Data Sharing Statement

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

Ethics Approval

This study was approved by the Ethics Committee of Guangxi Medical University Cancer Hospital (LW2021023), in compliance with the Declaration of Helsinki.

Author Contributions

All authors made a significant contribution to the work reported, whether that is in the conception, study design, execution, acquisition of data, analysis and interpretation, or in all these areas; took part in drafting, revising or critically reviewing the article; gave final approval of the version to be published; have agreed on the journal to which the article has been submitted; and agree to be accountable for all aspects of the work.

Disclosure

The authors declare that they have no conflict of interest.