Disulfiram Inhibits Epithelial–Mesenchymal Transition Through TGFβ–ERK–Snail Pathway Independently of Smad4 to Decrease Oral Squamous Cell Carcinoma Metastasis [Corrigendum]

This article refers to:

Disulfiram inhibits epithelial–mesenchymal transition through TGFβ–ERK–Snail pathway independently of Smad4 to decrease oral squamous cell carcinoma metastasis

Bu W, Wang Z, Meng L, et al. Cancer Manag Res. 2019;11:3887–3898.

The authors have advised due to an error at the time of figure assembly, Figures 2 and 3 on pages 3892 and 3893, respectively are incorrect. The correct Figures are as follows.

Figure 2 Dsf suppressed TGFβ₁-induced migration and invasion in OSCC cells.

Notes: (A and E) Transwell-based migration assays were performed to check the effect of Dsf on cell migration of Smad4-mutated CAL27 and Smad4 wild-type SCC25 cells exposed to TGFβ₁ (10 ng/mL). (B and F) Quantification of cell migration. (C and G) Transwell-based invasion assays were performed to check the effect of Dsf on cell invasion of Smad4-mutated CAL27 and Smad4 wild-type SCC25 cells exposed to TGFβ₁ (10 ng/mL). (D and H) Quantification of cell invasion. Values represent mean ± SEM. *P<0.05; **P<0.01; ***P<0.001; bar 300 μm.

Abbreviation: Dsf, disulfiram; OSCC, oral squamous cell carcinoma.

Figure 3 Dsf regulated EMT of OSCC cells through TGFβ–ERK–Snail pathway independently of Smad4.

Notes: (A and D) Western blot results of p-ERK and ERK derived from Smad4-mutated CAL27 and Smad4 wild-type SCC25 stimulated by TGFβ₁ for different periods (5, 15, 30, 60 minutes) or combined with Dsf (20 μM) pretreatment for 24 hours; quantitative results of Western blot from three independent experiments shown as mean ± SEM. Direct role of TGFβ–ERK–Snail pathway investigated using U0126, a chemical inhibitor of MEK1/2 and upstream activator of ERK. (B and E) Transwell-based migration and invasion assays were performed after cells had been exposed to TGFβ₁ (10 ng/mL) alone or combined with U0126 (20μM); quantification of cell migration and invasion shown as means ± SEM. (C and F) Western blot results of p-ERK, ERK, E-cadherin, vimentin and Snail from cells exposed to TGFβ₁ (10 ng/mL) alone or combined with U0126 (20 μM); quantitative results of Western blot from three independent experiments shown as mean ± SEM. *P<0.05; **P<0.01; ***P<0.001; bar 200 μm.

Abbreviations: Dsf, disulfiram; OSCC, oral squamous cell carcinoma; EMT, epithelial–mesenchymal transition.

The authors apologize for this error and advise it does not affect the results of the paper.