The mediating role of cytokine IL-6 on the relationship of FEV₁ upon 6-minute walk distance in chronic obstructive pulmonary disease

Abstract

Objectives

To explore the mediating role of protein interleukin-6 (IL-6) on the relationship between forced expiratory volume in 1 second (FEV₁) and 6-minute walk distance (6MWD) and, further, to determine whether status variables (such as age, sex, and body mass index [BMI]) operate as moderators of this mediation relationship.

Design

Moderated mediation model.

Setting

An inpatient pulmonary rehabilitation center in Italy.

Participants

All 153 patients involved in the screening of a randomized controlled clinical trial (ClinicalTrials.gov identifier: NCT1253941) were included in this study. All patients were Global initiative for chronic Obstructive Lung Disease (GOLD) stages I–IV and were aged 70.1±9.1 years.

Measurements

At run-in phase of the protocol, clinical and functional screening included BMI, fasting plasma levels of protein (IL-6), spirometry, and standardized 6-minute walking test, measured at the start of the respiratory rehabilitation program.

Methods

The size of the indirect effect of the initial variable (FEV₁) upon the outcome variable (6MWD) through the intervening variable (IL-6) was computed and tested for statistical significance. Moderated mediation analyses were subsequently conducted with age, sex, and BMI.

Results

FEV₁ averaged 53.4%±21.2%, and 6MWD 66.4%±41.3% of predicted. Median protein IL-6 was 6.68 pg/mL (interquartile range: 5.96). A bootstrapped mediation test supported the predicted indirect pathway (P=0.003). The indirect effect through IL-6 log units accounted for 17% of the total effect between FEV₁ and 6MWD. Age functioned as a significant moderator of the mediational pattern. For individuals aged <70 years, the standardized indirect effect was significant (0.122, 95% confidence interval [CI]: 0.044–0.254, P=0.004), and for individuals >70 years it was not significant (0.04, 95% CI: –0.010 to 0.142, P=0.10).

Conclusion

This moderated mediation result based on concurrent data suggests, but does not prove, a causal role of systemic inflammatory syndrome on progression from functional impairment to “frailty” status and substantial disability in aging chronic obstructive pulmonary disease.

Keywords:

• COPD
• chronic airflow obstruction
• aging
• mediation analysis

Acknowledgments

The authors acknowledge Dr Paola Abelli and Dr Rosanna Catella, medical directors at the Scientific Institute of Montescano. The authors would also like to thank the patients who participated in the study.

Disclosure

The authors report no conflicts of interest in this work.