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Infection and Drug Resistance Volume 14, 2021 - Issue
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Original Research

Expanding of ST11 Carbapenemase-Producing Klebsiella pneumoniae Subclones in a Chinese Hospital, Shenzhen, China

Min Zhang1 Shenzhen People’s Hospital, Shenzhen Institute of Respiratory Diseases, Shenzhen, Guangdong, People’s Republic of China;2 Bacteriology & Antibacterial Resistance Surveillance Laboratory, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong, People’s Republic of China
,
Jia Li3 Department of Nosocomial Infection Control, Bengbu Third People’s Hospital, Bengbu, People’s Republic of China
,
Yuemei Lu4 Department of Clinical Microbiology, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong, People’s Republic of China
,
Weiyuan Wu4 Department of Clinical Microbiology, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong, People’s Republic of China
,
Jinsong Wu4 Department of Clinical Microbiology, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong, People’s Republic of China
,
Yanji Xu1 Shenzhen People’s Hospital, Shenzhen Institute of Respiratory Diseases, Shenzhen, Guangdong, People’s Republic of China;5 Nanshan District Center of Chronic Disease Control, Shenzhen, People’s Republic of China
,
Yuxin Zhong1 Shenzhen People’s Hospital, Shenzhen Institute of Respiratory Diseases, Shenzhen, Guangdong, People’s Republic of China;2 Bacteriology & Antibacterial Resistance Surveillance Laboratory, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong, People’s Republic of China
,
Shiyi Liu1 Shenzhen People’s Hospital, Shenzhen Institute of Respiratory Diseases, Shenzhen, Guangdong, People’s Republic of China;2 Bacteriology & Antibacterial Resistance Surveillance Laboratory, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong, People’s Republic of China
,
Chuchu Lin6 Department of Nosocomial Infection Control, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong, People’s Republic of China
,
Shuye Xu3 Department of Nosocomial Infection Control, Bengbu Third People’s Hospital, Bengbu, People’s Republic of China
,
Dandan Chen1 Shenzhen People’s Hospital, Shenzhen Institute of Respiratory Diseases, Shenzhen, Guangdong, People’s Republic of China;2 Bacteriology & Antibacterial Resistance Surveillance Laboratory, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong, People’s Republic of China
,
Xuefeng Gao7 Department of Gastroenterology and Hepatology, Shenzhen University General Hospital, Shenzhen, People’s Republic of China
,
Yutian Luo8 Department of Intensive Care Unit, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong, People’s Republic of ChinaORCID Iconhttps://orcid.org/0000-0002-4943-5078
ORCID Icon,
Huaisheng Chen8 Department of Intensive Care Unit, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong, People’s Republic of China
,
Xueyan Liu8 Department of Intensive Care Unit, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong, People’s Republic of China
&
Wei Huang1 Shenzhen People’s Hospital, Shenzhen Institute of Respiratory Diseases, Shenzhen, Guangdong, People’s Republic of China;2 Bacteriology & Antibacterial Resistance Surveillance Laboratory, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong, People’s Republic of ChinaCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0002-8569-5184
ORCID Icon show all
Pages 1415-1422 | Published online: 13 Apr 2021
 
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Abstract

Background

ST11 is the most prevalent sequence type of clinical Klebsiella pneumoniae in China.

Methods

We investigated the characteristics of the ST11 subclones using core genome multi-locus sequence typing (cgMLST). Ninety-three carbapenemase-producing K. pneumoniae isolates were collected at Shenzhen People’s Hospital. Then, whole-genome sequencing and cgMLST were used to discriminate apparent subclones within the ST11 group.

Results

We analyzed the prevalence and genetic relationships of these subclones. ST11 and K. pneumoniae carbapenemase (KPC-2) were the predominant genotype and carbapenemase, respectively, in the clinical carbapenemase-producing K. pneumoniae strains. cgMLST scheme genotyping divided the ST11 group into two clades across seven complex types (CTs). CT1313 was the most prevalent subclone. The deletion of galF and a high frequency of SNPs in genes associated with the stress- and SOS-responses were found in CT1291 and CT2405 over time, respectively.

Conclusion

Our results indicated that the subclones of the ST11 group had different patterns of prevalence. Highly discriminatory genotyping techniques, such as cgMLST scheme, should be used in further molecular epidemiology investigations.

Data Sharing Statement

The genome sequences were deposited in the GenBank, under BioProject PRJNA517992.

Ethics Statement

This study was conducted in accordance with the Declaration of Helsinki. The research protocol was approved by the research ethics committee of Shenzhen People’s Hospital (reference 257 number LL-KY-2019002). The research ethics committee of Shenzhen People’s Hospital did not require written informed consent from participants because no personal information about patients was requested.

Author Contributions

All authors made a significant contribution to the work reported, whether that is in the conception, study design, execution, acquisition of data, analysis and interpretation, or in all these areas; took part in drafting, revising or critically reviewing the article; gave final approval of the version to be published; have agreed on the journal to which the article has been submitted; and agree to be accountable for all aspects of the work.

Disclosure

The authors state that they have no conflicts of interest.

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