Direct-on-Target Microdroplet Growth Assay Applications for Clinical Antimicrobial Susceptibility Testing

Abstract

Direct-on-target microdroplet growth assay is a new technique for analysing bacterial sensitivity and mechanisms of resistance. It is based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and allows for easy and rapid testing. Here, we describe the development and procedure of the direct-on-target microdroplet growth assay and summarise the latest clinical applications.

Keywords:

• matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
• MALDI-TOF MS
• direct-on-target microdroplet growth assay
• DOT-MGA
• antimicrobial susceptibility testing

Data Sharing Statement

The authors declare that all data and materials support their published claims and comply with field standards.

Author Contributions

All authors made substantial contributions to conception and design, acquisition of data, or analysis and interpretation of data; took part in drafting the article or revising it critically for important intellectual content; agreed to submit to the current journal; gave final approval of the version to be published; and agree to be accountable for all aspects of the work.

Disclosure

The authors declare that they have no conflict of interest.