Antibiotic Resistance Genes Among Carbapenem-resistant Enterobacterales (CRE) Isolates of Prapokklao Hospital, Chanthaburi Province, Thailand

Abstract

Background

The global spread of carbapenem-resistant Enterobacterales (CRE) inflicts a severe threat to human health. The CRE infections have resulted in an increased mortality rate in hospitals and other health-care settings worldwide. In this study, the antibiotic-resistance pattern and prevalence of carbapenemase-encoding genes among CRE isolated from patients of one hospital in Thailand were investigated.

Methods

By using conventional biochemical tests, we identified and isolated all species of Enterobacterales from the clinical samples kept at Prapokklao Hospital, Chanthaburi, Thailand, which were collected during 2016–2017. Multidrug-resistant (MDR) bacteria were determined by disc diffusion method and minimum inhibitory concentration (MIC) test strips. Carbapenemase genes were detected by PCR and confirmed by Sanger sequencing.

Results

Klebsiella pneumoniae complex, Escherichia coli, and Enterobacter spp. were isolated from the specimens. Of 9,564 isolated Enterobacterales, 282 were multidrug-resistance (MDR). The MIC test strips revealed that the MDR CRE were resistant to ertapenem (92.9%) and meropenem (81.3%). All these isolates carried carbapenemase-coding genes, including bla_NDM (90%) and bla_IMP (71%), the two most commonly found genes among CRE strains. There were 39.2% of the isolates that carried a combination of bla_NDM-bla_IMP and 22.6% carried combined bla_NDM-bla_IMP-bla_OXA-48-like genes.

Conclusion

This study demonstrates a significantly high prevalence of CRE isolates with the MDR phenotypes. A minority of the isolates carried a single carbapenem-resistant gene, while the majority harbored multiple genes in combination. Regular monitoring of MDR CRE and characterization of their drug resistance are important for guiding treatment, intervention and control of the CRE spread and outbreak in a health-care setting.

Keywords:

• carbapenemase
• CRE
• drug resistant
• Enterobacterales
• nosocomial infections

Acknowledgments

This work was supported by the Tropical Medicine Grants 2017 from Faculty of Tropical Medicine, Mahidol University to Witawat Tunyong. We would like to thank Mr Paul Adams, Research Administrator of Faculty of Tropical Medicine, Mahidol University, for editing English of this manuscript.

Disclosure

The authors report no conflicts of interest in this work.