Genetic Characterization of bla_NDM-1-Carrying Citrobacter portucalensis Sequence Type 328 and Citrobacter freundii Sequence Type 98

Abstract

Purpose

NDM-1-producing Citrobacter portucalensis and Citrobacter freundii simultaneously occurred in a hospital. This study aims to characterize the bla_NDM-1-carrying plasmids in these Citrobacter strains.

Methods

Cf7303, Cf7308, and Cf7313 were recovered from three patients in a teaching hospital from September 24 to October 1, 2021. Bacteria were identified by MALDI-TOF mass spectrometry, and antibiotics susceptibility tests were determined by VITEK^® 2 compact system. Whole-genome sequencing (WGS) was performed using the HiSeq Illumina and QNome platform to characterize the genomes.

Results

Cf7303 was identified as C. portucalensis Sequence Type 328 by WGS, and harbored two plasmids, namely pCf7303 and a novel IncFIB pNDM-Cf7303 on which antibiotic-resistant genes (bla_TEM-1, bla_CTX-M-14, bla_NDM-1, aac (3)-IId, aadA2, fosA3, sul1, sul2, catA2, tetD, dfrA12, qacEdelta1, mph(A), and ble_MBL) are located. C. freundii strain Cf7308 and Cf7313 belonged to the same Sequence Type 98. Cf7308 contained two plasmids, pCf7308, and an IncN1 pNDM-Cf7308 with homology to pNDM-BTR in E. coli and pNDM-CWH001 in C. freundii.

Conclusion

We characterized a putatively novel IncFIB plasmid carrying bla_NDM-1 in C. portucalensis. In addition, the closely related bla_NDM-1-carrying IncN1 plasmids in E. coli and C. freundii suggest that interspecies or intraspecies horizontal transfer occurs in China.

Keywords:

• NDM-1
• Citrobacter portucalensis
• Citrobacter freundii
• whole-genome sequencing

Ethical Approval Statements

This retrospective study was approved by the Evaluation Committee and the Biomedical Ethics Committee of Beijing Tsinghua Changgung Hospital (22031-0-01). Because of the retrospective and anonymous nature of the study, the Ethics Committee did not require written informed consent provided by participants.

Disclosure

Yu He is affiliated with Qitan Technology Ltd. All authors declare no other conflicts of interest in this work.