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Abstract
Purpose
Beta-lactamase-producing Klebsiella pneumoniae is common in the clinic, but research associated with the co-existence of KPC-2, LAP-2, and CTX-M-65 in K. pneumoniae is still rare. In this study, the phenotypic and genetic characteristics of a multidrug-resistant K. pneumoniae strain SJ25 co-harboring blaKPC-2, blaLAP-2, and blaCTX-M-65 with rare ST1469 were investigated.
Methods and Results
Antimicrobial susceptibility testing revealed that strain SJ25 was resistant to various common antibiotics, except ciprofloxacin, fosfomycin, colistin, and tigecycline. Whole-genome analysis revealed that strain SJ25 carries a variety of antimicrobial resistance genes and virulence determinants. Plasmid analysis confirmed that the blaKPC-2 and blaCTX-M-65 genes were located on an ~136 kb transferrable IncFII/IncR plasmid and that blaLAP-2 was located on an untypeable plasmid.
Conclusion
Our findings emphasized the need for continuous surveillance of β-lactamase-bearing K. pneumoniae in the clinic to control potential dissemination and outbreak.
Nucleotide Sequence Accession Numbers
The whole-genome sequences of the K. pneumoniae were submitted to GenBank under the following BioProject number: PRJNA885055.
Ethics Approval and Consent to Participate
This study was conducted following the Declaration of Helsinki and obtained approval from the clinical research ethics committee of the First Affiliated Hospital, Zhejiang University School of Medicine [number 2020-IIT-660]. The patient provided written informed consent to allow the case details to be published.
Acknowledgments
This work was supported by research grants from the National Natural Science Foundation of China (No. 82072314) and the Research Project of Jinan Microecological Biomedicine Shandong Laboratory (JNL-2022011B).
Disclosure
The authors report no conflicts of interest in this work.