Abstract
Background
Proteins contained in the conserved YTH521-b homologous (YTH) domain, have m6A-dependent RNA binding activity. As an important part of YTH domain family proteins, YTHDF1 and YTHDF3 were shown to be associated with many cancers. This paper aimed to reveal the relationship between the expression of these two proteins and the clinical prognosis of OSCC, providing certain guidance for clinical treatment of OSCC.
Methods
We detected the expression of YTHDF1 and YTHDF3 in 120 OSCC patients by immunohistochemical analysis. Statistical analysis was used to determine whether the high or low expression of these two genes was significantly associated with age, gender, histological type, clinical stage, or lymph node metastasis. The correlation curve and survival curve of the two genes were produced to evaluate the potential clinical significance.
Results
We find the expression of YTHDF1 and YTHDF3 was increased in OSCC tissues compared to adjacent normal tissues. The statistical analysis showed that the expression of YTHDF1 and YTHDF3 was significantly associated with the clinical stage and histological type in OSCC patients. There was also a significant correlation between the expression of YTHDF1 and YTHDF3. A high expression of YTHDF1 and YTHDF3 was related to poor patient prognosis.
Conclusion
Our findings suggest that a high expression of YTHDF1 and YTHDF3 may be related to poor patient prognosis.
Data Sharing Statement
The datasets used or analysed during the current study available from the corresponding author on reasonable request.
Ethics Statement and Consent to Participant
The project was approved by the Qingdao University affiliated hospital (QYFYMZJJ26812). All methods were performed in accordance with the relevant guidelines and regulations.The ethics committee of the institute agree informed consent because the data that were collected were secondary data. The investigation conforms to the principles outlined in the declaration of Helsinki.
Disclosure
Rui Shi, Shao-ming Li, Ling Gao have contributed equally to this work, to be the co-first authors. The authors declare no conflict of interest.