Gold nanoparticle–choline complexes can block nicotinic acetylcholine receptors

Abstract

We identified a novel class of direct ion-channel blockers of ligand-gated ion channels called the gold nanoparticle–choline complex. Negatively charged gold nanoparticles (1.4 nm) block ion pores by binding to the sulfur group of the cysteine loop of nicotinic acetylcholine receptors (nAChRs), and currents evoked by acetylcholine (Ach) can break these bonds. The current evoked by ACh in nAChRs was blocked directly in ion pores by the gold nanoparticle– choline complex. In adrenal-gland perfusion studies, the complex also blocked nAChRs by diminishing catecholamine release by about 75%. An in vivo study showed muscle relaxation in rats after injection of the complex. These results will foster the application of gold nanoparticles as a direct ion-channel blocker.

Keywords:

• negatively charged gold nanoparticle
• choline
• gold–sulfur bond
• nicotinic acetylcholine receptor
• direct ion-channel blocker

Disclosure

The authors report no conflicts of interest in this work.