A highly sensitive biosensor based on Au NPs/rGO-PAMAM-Fc nanomaterials for detection of cholesterol

Jianmeng Zhu1 Department of Clinical Laboratory, Chun’an First People’s Hospital (Zhejiang Provincial People’s Hospital Chun’an Branch), Hangzhou, Zhejiang, China, [email protected], [email protected]

Zhilu Ye1 Department of Clinical Laboratory, Chun’an First People’s Hospital (Zhejiang Provincial People’s Hospital Chun’an Branch), Hangzhou, Zhejiang, China, [email protected], [email protected]

Xiaoying Fan1 Department of Clinical Laboratory, Chun’an First People’s Hospital (Zhejiang Provincial People’s Hospital Chun’an Branch), Hangzhou, Zhejiang, China, [email protected], [email protected]

Hongqin Wang1 Department of Clinical Laboratory, Chun’an First People’s Hospital (Zhejiang Provincial People’s Hospital Chun’an Branch), Hangzhou, Zhejiang, China, [email protected], [email protected]

Zhen Wang1 Department of Clinical Laboratory, Chun’an First People’s Hospital (Zhejiang Provincial People’s Hospital Chun’an Branch), Hangzhou, Zhejiang, China, [email protected], [email protected];2 Department of Blood Transfusion, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China, [email protected], [email protected]Correspondence[email protected]

Bingyu Chen1 Department of Clinical Laboratory, Chun’an First People’s Hospital (Zhejiang Provincial People’s Hospital Chun’an Branch), Hangzhou, Zhejiang, China, [email protected], [email protected];2 Department of Blood Transfusion, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China, [email protected], [email protected]Correspondence[email protected]

Abstract

Objective

This study aimed to construct a biosensor using Au nanoparticles (Au NPs) and reduced graphene-polyamide-amine-ferrocene (rGO-PAMAM-Fc) nanomaterials designed for rapid and sensitive detection of cholesterol.

Materials and methods

In this study, a highly sensitive biosensor based on Au NPs/ rGO-PAMAM-Fc nanomaterials was manufactured for detection of cholesterol. The rGO-PAMAM-Fc and Au NPs were modified on the surface of the electrode and then coated with cholesterol oxidase (ChOx) and cholesterol esterase (ChEt) to develop the ChOx&ChEt/Au NPs/rGO-PAMAM-Fc biosensor.

Results

The capability of rGO-PAMAM-Fc nanomaterials in fabricating a more efficient biosensor was validated through stability, selectivity and reproducibility checks. Under optimal conditions, the newly developed biosensor showed a linear relationship with logarithm of cholesterol concentration from 0.0004 to 15.36 mM (R²=0.9986), and a low detection limit of 2 nM was obtained at the signal/noise ratio of 3.

Conclusion

The ChOx&ChEt/Au NPs/rGO-PAMAM-Fc biosensor was successfully applied for the measurement of cholesterol in human serum, which implies that the biosensor has a potential application in clinical diagnostics.

Keywords:

Acknowledgments

This work is supported by the Health Science and Technology Project of Hangzhou (2018B072), Medicine and Health Research Foundation of Zhejiang Province (2018KY147, 2019KY017, 2016DTB001, 2017KY005), the Basic Ability Enhancement Program for Young and Middle-age Teachers of Guangxi (2018KY0115), Fund for “Integration of Cloud Computing and Big Data, Innovation of Science and Education” (2017A11036), Traditional Chinese Medicine Scientific Research Foundation of Zhejiang Province (2018ZY001, 2019ZZ001), Natural Science Foundation of Zhejiang Province (Y15H280010, LQ17H160017) and Hangzhou Science Technology Bureau (20140633B67).

Disclosure

The authors report no conflicts of interest in this work.

A highly sensitive biosensor based on Au NPs/rGO-PAMAM-Fc nanomaterials for detection of cholesterol

Objective

Materials and methods

Results

Conclusion

Information for

Open access

Opportunities

Help and information

A highly sensitive biosensor based on Au NPs/rGO-PAMAM-Fc nanomaterials for detection of cholesterol

Abstract

Objective

Materials and methods

Results

Conclusion

Acknowledgments

Disclosure

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