https://orcid.org/0000-0002-0411-9557
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Abstract
Background:
Kaempferol (K) is a recognized anticancer drug that can conjugate with small-size gold nanoclusters (AuNCs).
Materials and methods:
K-AuNCs were synthesized and their use as an anticancer drug was explored using A549 lung cancer cells. Colony formation and cell migration assays were carried out. The morphology of the K-AuNCs treated A549 cells was explored using bio-atomic force microscopy.
Results:
The K-AuNCs were 1-3 nm in diameter and emitted strong fluorescent at 650 nm following excitation at 550 nm. The stretching and bending nature of the K-AuNCs were analyzed by the Fourier transform infrared spectroscopy. The presence of kaempferol in the AuNCs were confirmed by the PL spectroscopy.
Conclusion:
The synthesized K-AuNCs mainly targeted and damaged the nuclei of the cancer cells. This composite nanocluster was less toxicity to the normal human cell and higher toxicity to the A549 lunch cancer cell and these material is potential for anticancer drug delivery and bio imaging applications.
Acknowledgments
This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (No.2017R1A2B4004700). This work was supported by the Ministry of Trade, Industry, and Energy (Grant No.20000580).
Disclosure
The authors report no conflicts of interest in this work.
Supplementary materials
Figure S1 Fluorescence stability of the K-AuNCs was checked with six months before synthesized sample, and it was stored in 4°C. The synthesized material gives good fluorescence intensity. The inset image indicated that red color fluorescence is present under the UV light for the same sample
Figure S2 (A) Annixin V-FITC/PI double staining of apoptosis in A549 control or K-AuNCs (12.5 µg/mL) treated cells. (B) Table represents the percentage of cells live and in different stages of apoptosis
