Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells

Lei Wang1 Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China;2 Faculty of Oncology, Medical School, Southeast University, Nanjing, China

Haijun Zhang1 Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China;2 Faculty of Oncology, Medical School, Southeast University, Nanjing, China

Baoan Chen1 Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China;2 Faculty of Oncology, Medical School, Southeast University, Nanjing, ChinaCorrespondence[email protected]

Guohua Xia1 Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China;2 Faculty of Oncology, Medical School, Southeast University, Nanjing, China

Shuai Wang1 Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China;2 Faculty of Oncology, Medical School, Southeast University, Nanjing, China

Jian Cheng1 Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China;2 Faculty of Oncology, Medical School, Southeast University, Nanjing, China

Zeye Shao1 Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China;2 Faculty of Oncology, Medical School, Southeast University, Nanjing, China

Chong Gao1 Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China;2 Faculty of Oncology, Medical School, Southeast University, Nanjing, China

Wen Bao1 Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China;2 Faculty of Oncology, Medical School, Southeast University, Nanjing, China

Liang Tian1 Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China;2 Faculty of Oncology, Medical School, Southeast University, Nanjing, China

Yanyan Ren1 Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China;2 Faculty of Oncology, Medical School, Southeast University, Nanjing, China

Peipei Xu1 Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China;2 Faculty of Oncology, Medical School, Southeast University, Nanjing, China

Xiaohui Cai1 Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China;2 Faculty of Oncology, Medical School, Southeast University, Nanjing, China

Ran Liu1 Department of Hematology and Oncology, Zhongda Hospital, Medical School, Southeast University, Nanjing, China;2 Faculty of Oncology, Medical School, Southeast University, Nanjing, China

Xuemei Wang3 State Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing, China

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Abstract

Traditional Chinese medicine is gradually becoming a new source of anticancer drugs. One such example is wogonin, which is cytotoxic to various cancer cell lines in vitro. However, due to its low water solubility, wogonin is restricted to clinical administration. Recently, the application of drug-coated magnetic nanoparticles (MNPs) to increase water solubility of the drug and to enhance its chemotherapeutic efficiency has attracted much attention. In this study, wogonin was conjugated with the drug delivery system of MNPs by mechanical absorption polymerization to fabricate wogonin-loaded MNPs. It was demonstrated that MNPs could strengthen wogonin-induced cell inhibition, apoptosis, and cell cycle arrest in Raji cells by methylthiazol tetrazolium assay, flow cytometer assay, and nuclear 4′,6-diamidino-2-phenylindole staining. Furthermore, the molecular mechanisms of these phenomena were explored by western blot, in which the protein levels of caspase 8 and caspase 3 were increased significantly while those of survivin and cyclin E were decreased significantly in wogonin-MNPs group. These findings suggest that the combination of wogonin and MNPs provides a promising strategy for lymphoma therapy.

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Acknowledgments

This work was financially supported by National Key Basic Research Program 973 of China (No 2010CB732404), National Natural Science Funds of People’s Republic of China (No 81170492), and Key Medical Disciplines of Jiangsu Province.

Disclosure

The authors report no conflicts of interest in this work.

Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells

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Effect of magnetic nanoparticles on apoptosis and cell cycle induced by wogonin in Raji cells

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