Efficacy and safety of nanohybrids comprising silver nanoparticles and silicate clay for controlling Salmonella infection

Shu-Her Chiao1 Department of Life Sciences, Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan

Siou-Hong Lin1 Department of Life Sciences, Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan

Ching-I Shen2 Department of Chemistry, Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan

Jiunn-Wang Liao3 Graduate Institute of Veterinary Pathobiology, Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan

I-Jiuan Bau1 Department of Life Sciences, Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan

Jiun-Chiou Wei4 Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan

Li-Ping Tseng1 Department of Life Sciences, Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan

Shan-hui Hsu4 Institute of Polymer Science and Engineering, National Taiwan University, Taipei, Taiwan

Ping-Shan Lai2 Department of Chemistry, Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan

Shinn-Zong Lin5 Center for Neuropsychiatry, China Medical University and Hospital, Taichung, Taiwan;6 Department of Neurosurgery, China Medical University Beigan Hospital, Yunlin, Taiwan;7 Graduate Institute of Immunology, China Medical University, Taichung, Taiwan

Jiang-Jen Lin4 Institute of Polymer Science and Engineering, National Taiwan University, Taipei, TaiwanCorrespondence[email protected] [email protected]

Hong-Lin Su1 Department of Life Sciences, Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan;8 Department of Physical Therapy, China Medical University, Taichung, TaiwanCorrespondence[email protected] [email protected]

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Abstract

Developing effective and safe drugs is imperative for replacing antibiotics and controlling multidrug-resistant microbes. Nanoscale silicate platelet (NSP) and its nanohybrid, silver nanoparticle/NSP (AgNP/NSP), have been developed, and the nanohybrids show a strong and general antibacterial activity in vitro. Here, their efficacy for protecting Salmonella-infected chicks from fatality and septicemia was evaluated. Both orally administrated NSP and AgNP/NSP, but not AgNPs alone, effectively reduced the systemic Salmonella infection and mortality. In addition, quantitative Ag analyses demonstrated that Ag deposition from AgNP/NSP in the intestines was less than that from conventional AgNPs, indicating that the presence of NSP for immobilizing AgNPs reduced Ag accumulation in tissue and improved the safety of AgNPs. These in vivo results illustrated that both NSP and AgNP/NSP nanohybrid represent potential agents for controlling enteric bacterial infections.

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Acknowledgments

This work was supported by the National Research Program for Nanoscience and Technology sponsored by the National Science Council (100-2120-M-002-006). The research was also funded in part by the Ministry of Education, Taiwan, Republic of China, under the ATU Plan. Assistance provided by Dr Cheng-Chung Lin at the Graduate Institute of Veterinary Pathobiology, NCHU is highly appreciated.

Disclosure

The authors declare no conflicts of interest in this work.

Supplementary materials

Table S1 Histopathological findings in the nanomaterial-fed chicks

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Figure S1 The antimicrobial effect of the silver nanoparticle (AgNPs). Approximately 1 × 10³ Salmonella pullorum and Escherichia coli, synchronized at the log phase of the growth curve, were spread on Luria-Bertani agars where AgNPs or AgNP/nanoscale silicate platelet (NSP) were mixed with at the indicated concentrations.

Note: The numbers of colonies were counted after overnight incubation at 37°C.

Efficacy and safety of nanohybrids comprising silver nanoparticles and silicate clay for controlling Salmonella infection

Table S1 Histopathological findings in the nanomaterial-fed chicks

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Efficacy and safety of nanohybrids comprising silver nanoparticles and silicate clay for controlling Salmonella infection

Abstract

Acknowledgments

Disclosure

Supplementary materials

Table S1 Histopathological findings in the nanomaterial-fed chicks

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