Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity

Luiz Cláudio da Silva1 Drug Department, Rio de Janeiro Federal University, Rio de Janeiro, BrazilCorrespondence[email protected]

Thiago Garcia1 Drug Department, Rio de Janeiro Federal University, Rio de Janeiro, Brazil

Michela Mori2 Department of Drug Sciences, University of Pavia, Pavia, Italy

Giuseppina Sandri2 Department of Drug Sciences, University of Pavia, Pavia, Italy

Maria Cristina Bonferoni2 Department of Drug Sciences, University of Pavia, Pavia, Italy

Priscila V Finotelli1 Drug Department, Rio de Janeiro Federal University, Rio de Janeiro, Brazil

Leonardo P Cinelli1 Drug Department, Rio de Janeiro Federal University, Rio de Janeiro, Brazil

Carla Caramella2 Department of Drug Sciences, University of Pavia, Pavia, Italy

Lúcio M Cabral1 Drug Department, Rio de Janeiro Federal University, Rio de Janeiro, Brazil

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Abstract

The aim of this study was to produce and characterize nanoparticles (NPs), combining chondroitin sulfate (CS) and fucoidan (FC) with chitosan for therapeutic purposes. These NPs were characterized by dynamic light scattering, zeta potential determination, and transmission electronic microscopy. The anticoagulant activity was determined for FC NPs and compared with FC solution at the same concentration. FC NPs showed regular shapes and better anticoagulant activity than free polysaccharide solution. FC solution did not affect coagulation compared to FC NPs, which increased up to two-fold, even at a lower concentration. Cytotoxicity and permeability tests were conducted using Caco-2 cell monolayer, exhibiting no toxic effect in this cell line and higher permeability for NP2 samples than FC solution at the same concentration.

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Acknowledgments

This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (6711/10-3). Edital CAPES Nanobiotecnologia 2008.

Disclosure

The authors report no conflicts of interest in this work.

Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity

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Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity

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