Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold

Lu Cao1 Department of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China;2 State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, China

Ping-Guo Duan1 Department of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China;2 State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, China

Hui-Ren Wang1 Department of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China;2 State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, China

Xi-Lei Li1 Department of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China;2 State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, China

Feng-Lai Yuan3 Affiliated Third Hospital of Nantong University, Wuxi, Jiangsu, China

Zhong-Yong Fan4 Department of Materials Science, Fudan University, Shanghai, China

Su-Ming Li5 Max Mousseron Institute on Biomolecules, Montpellier I University, Montpellier, FranceCorrespondence[email protected] [email protected]

Jian Dong1 Department of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China;2 State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai, ChinaCorrespondence[email protected] [email protected]

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Abstract

The purpose of this study was to investigate the influence of nano-sized β-tricalcium phosphate (β-TCP) on the biological performance of poly (lactic acid) (PLA) composite scaffolds by using in vitro degradation and an in vivo model of heterotopic bone formation. Nano-sized β-TCP (nβ-TCP) was prepared with a wet grinding method from micro-sized β-TCP (mβ-TCP), and composite scaffolds containing 0, 10, 30, or 50 wt% nβ-TCP or 30 wt% mβ-TCP were generated using a freeze-drying method. Degradation was assessed by monitoring changes in microstructure, pH, weight, and compressive strength over a 26-week period of hydrolysis. Composite scaffolds were processed into blocks, and implanted into muscular pockets of rabbits after loading with recombinant human bone morphogenetic protein-2 (rhBMP-2). New bone formation was evaluated based on histological and immunohistochemical analysis 2, 4, and 8 weeks after implantation. The in vitro results indicated that the buffering effect of nβ-TCP was stronger than mβ-TCP, which was positively correlated with the content of nβ-TCP. The in vivo findings demonstrated that nβ-TCP enhanced the osteoconductivity of the scaffolds. Although composite scaffolds containing 30% nβ-TCP exhibited similar osteoconductivity to 50% nβ-TCP, they had better mechanical properties than the 50% nβ-TCP scaffolds. This study supports the potential application of a composite scaffold containing 30% nβ-TCP as a promising scaffold for bone regeneration.

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Acknowledgments

This work was supported by the National Basic Research Program of China (973 program) (2009CB930002), Shanghai International Science and Technology Partnership Program (11540702700), Natural Science Foundation of China (31170925), and the National Natural Science Foundation of China (30970718).

Disclosure

The authors report no conflicts of interest in this work.

Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold

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Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold

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