Preparation, characterization, and in vitro release study of albendazole-encapsulated nanosize liposomes

Abstract

The purpose of the present study was to formulate effective and controlled release albendazole liposomal formulations. Albendazole, a hydrophobic drug used for the treatment of hydatid cysts, was encapsulated in nanosize liposomes. Rapid evaporation method was used for the preparation of albendazole-encapsulated conventional and PEGylated liposomes consisting of egg phosphatidylcholine (PC) and cholesterol (CH) in the molar ratios of (6:4) and PC:CH: polyethylene glycol (PEG) (5:4:1), respectively. In this study, PEGylated and conventional liposomes containing albendazole were prepared and their characteristics, such as particle size, encapsulation efficiency, and in vitro drug release were investigated. The drug encapsulation efficiency of PEGylated and conventional liposomes was 81% and 72%, respectively. The biophysical characterization of both conventional and PEG-coated liposomes were done by transmission electron microscopy and UV-vis spectrophotometry. Efforts were made to study in vitro release of albendazole. The drug release rate showed decrease in albendazole release in descending order: free albendazole, albendazole-loaded conventional liposomes, and least with albendazole-loaded PEG-liposomes. Biologically relevant vesicles were prepared and in vitro release of liposome-entrapped albendazole was determined.

Keywords:

• albendazole
• PEGylated
• in vitro release
• liposomes
• nanosize

Acknowledgements

The authors thank the technical support provided by State Biotechnology Programme, Biotech Bhawan, Haldi-263146, Uttarakhand. The authors also thank the Directorate of Research, G.B. Pant University of Agriculture and Technology, Pantnagar for providing laboratory facilities and Dr Rajendra Dobhal, Advisor, State Biotechnology Programme, Biotech Bhawan, Haldi-263146, Uttarakhand. The authors report no conflicts of interest in this work.