Characterization of nanostructured ureteral stent with gradient degradation in a porcine model

Abstract

A tubular poly(ε-caprolactone) (PCL)/poly(lactide-co-glycolide) (PLGA) ureteral stent composed of nanofibers with micropores was fabricated by double-needle electrospinning. The stent was ureteroscopically inserted into six Changbai pigs, and the commercial polyurethane Shagong^® stent was inserted into four pigs as control. Intravenous pyelography revealed that the PCL/PLGA stent gradually degraded from the distal end to proximal terminal, and all stents were completely degraded at 10 weeks post-insertion. No significant difference was observed in hydronephrosis severity between the two groups. The levels of serum creatinine and urine pH remained similar throughout the study in the two groups, but the number of white blood cells in the urine was significantly higher in the Shagong^® stent group. On Day 70, histological evaluation indicated equivalent histological severity scores in the middle and distal ureter sections and bladder in the two groups. However, the PCL/PLGA stent-implanted pigs had significantly lower mean severity scores in the kidney and proximal ureter sites. These data revealed that the PCL/PLGA stent degraded in a controlled manner, did not induce obstruction, and had a lower urothelial impact in comparison to the Shagong^® stent, indicating that the stent exhibited great potential for clinical application.

Keywords:

• nanocomposites
• polyesters
• poly(ε-caprolactone)
• poly(lactide-co-glycolide)
• hydronephrosis severity

Acknowledgments

This study was financially supported by the National Natural Science Foundation of China (numbers 50973043, 51303174, 51233004, and 51390484), the Development and Reform Commission of Jilin Province (number JF-2012C006-5), and the Scientific Development Program of Jilin Province (number 20120159).

Disclosure

The authors report no conflicts of interest in this work.