Assessing the axonal translocation of CeO₂ and SiO₂ nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study

Abstract

The axonal translocation of two commonly used nanoparticles in medicine, namely CeO₂ and SiO₂, is investigated. The study was conducted on frog sciatic nerve fibers in an ex vivo preparation. Nanoparticles were applied at the proximal end of the excised nerve. A nerve stimulation protocol was followed for over 35 hours. Nerve vitality curve comparison between control and exposed nerves showed that CeO₂ has no neurotoxic effect at the concentrations tested. After exposure, specimens were fixed and then screen scanned every 1 mm along their length for nanoparticle presence by means of Fourier transform infrared microscopy. We demonstrated that both nanoparticles translocate within the nerve by formation of narrow bands in the Fourier transform infrared spectrum. For the CeO₂, we also demonstrated that the translocation depends on both axonal integrity and electrical activity. The speed of translocation for the two species was estimated in the range of 0.45–0.58 mm/h, close to slow axonal transportation rate. Transmission electron microscopy provided direct evidence for the presence of SiO₂ in the treated nerves.

Keywords:

• CeO₂
• SiO₂
• FTIR
• nanoparticles
• ex vivo electrophysiology
• frog sciatic nerve
• translocation

Disclosure

The authors report no conflicts of interest in this work.