Phytofabrication of bioinduced silver nanoparticles for biomedical applications

Abstract

Synthesis of nanomaterials holds infinite possibilities as nanotechnology is revolutionizing the field of medicine by its myriad applications. Green synthesis of nanoparticles has become the need of the hour because of its eco-friendly, nontoxic, and economic nature. In this study, leaf extract of Rosa damascena was used as a bioreductant to reduce silver nitrate, leading to synthesis of silver nanoparticles (AgNPs) in a single step, without the use of any additional reducing or capping agents. The synthesized nanoparticles were characterized by the use of UV-visible spectroscopy, fourier transform infrared spectroscopy, dynamic light scattering, transmission electron microscopy, and field emission scanning electron microscopy. Time-dependent synthesis of AgNPs was studied spectrophotometrically. Synthesized AgNPs were found to possess flower-like spherical structure where individual nanoparticles were of 16 nm in diameter, whereas the agglomerated AgNPs were in the range of 60–80 nm. These biologically synthesized AgNPs exhibited significant antibacterial activity against Gram-negative bacterial species but not against Gram-positive ones (Escherichia coli and Bacillus cereus). Anti-inflammatory and analgesic activities were studied on a Wistar rat model to gauge the impact of AgNPs for a probable role in these applications. AgNPs tested positive for both these activities, although the potency was less as compared to the standard drugs.

Keywords:

• silver nanoparticles
• green synthesis
• anti-inflammatory
• analgesic
• animal model study
• antibacterial

Acknowledgments

The authors wish to acknowledge Prof RM Dubey, Vice Chancellor of the IFTM University, Moradabad; Prof AK Ghosh, Director, School of Biotechnology; Prof Sanjay Mishra, Dean, School of Biotechnology; and Mr Phool Chand, Assistant Professor, School of Pharmaceutical Sciences, IFTM University, Moradabad for providing all the necessary facilities, support, and encouragement to the authors to complete this task. We would also like to extend our gratitude to the SAIF Chandigarh, India for characterization of samples.

Disclosure

The authors report no conflicts of interest in this work.