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ORIGINAL RESEARCH

MicroRNA-146a-5p and microRNA‐210‐3p Correlate with T Regulatory Cells Frequency and Predict Asthma Severity in Egyptian Pediatric Population

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Pages 107-121 | Received 21 Nov 2022, Accepted 10 Jan 2023, Published online: 20 Jan 2023
 

Abstract

Background

Severe bronchial asthma (BA) affects 5–10% of children, which imposes socioeconomic burden. Therefore, it is crucial to identify biomarkers for risk stratification in children with BA. T regulatory cells (Tregs) play a balancing role in allergic response regulation. We aimed to investigate the relationship between Treg, miR-210-3p, and miR-146a-5p in relation to asthma phenotypes in search of novel biomarkers of disease severity.

Methods

This study included 50 children with BA classified into Group 1 (n = 25) children with mild to moderate asthma and Group 2 (n = 25) children with severe asthma. In addition to 26 control subjects. Flow cytometry was used to detect Tregs. Plasma miR-210-3p and miR-146a levels were determined using quantitative real-time PCR. Patients’ FEV1 (Forced Expiratory Volume in the first second) was measured.

Results

miR-210-3p level correlated negatively with Treg frequency (r = −0.828, P < 0.001) and FEV1 (r = −0.621, P < 0.001). The level of miR-146a-5p positively correlated positively with Treg% (r = 0.303, P = 0.032). ROC curve analysis revealed that miR-210-3p was the most sensitive biomarker of severity, with the area under curve (AUC) = 0.923, 96% sensitivity, and 60% specificity. According to multivariate analysis, miR-210-3p is an independent risk factor for BA severity [OR =3.119, P = 0.030], while miR-146a-5p is a protective factor [OR =0.811, P = 0.049].

Conclusion

Treg frequency is linked to FEV1, miR-146a-5p and miR-210-3p in childhood BA. Upregulation of miR-210-3p is a sensitive biomarker and an independent risk factor for BA severity in Egyptian children.

Data Sharing Statement

Data is available upon request.

Acknowledgment

We are grateful to all the patients who participated in the study.

Disclosure

The authors report no conflicts of interest in this work.