Therapeutic Effects of Spirulina platensis Against Adolescent Stress-Induced Oxidative Stress, Brain-Derived Neurotrophic Factor Alterations and Morphological Remodeling in the Amygdala of Adult Female Rats

Abstract

Objective

The amygdala structural and functional abnormalities have been implicated in numerous neuropsychiatric and neurodevelopmental disorders. Given the important role of the amygdala in stress responses and the susceptibility of the females to adolescent stress, the present study investigated the beneficial effects of Spirulina platensis microalgae (SP) as a neuroprotective supplement against adolescent stress-induced oxidative stress, brain-derived neurotrophic factor (BDNF) alterations, molecular and morphological remodeling in the basolateral amygdala (BLA) of adult female rats.

Methods

During the adolescent period (PNDs 30–40) rats were subjected to restraint stress (2 h/day for 10 days). Then, the animals were subjected to 15 days treatment (PNDs 41–55) with SP (200 mg/kg/day) followed by biochemical (BDNF and stress oxidative markers), molecular (BDNF and its receptor tropomyosin receptor kinase B [TrkB] mRNA expression), and morphological (dendritic length and spines) assessments in the BLA.

Results

The study revealed that adolescent stress decreased BDNF levels and reduced apical dendritic length and branch points of pyramidal neurons in the BLA. In addition, chronic stress significantly increased oxidative stress parameters and decreased BDNF and TrkB mRNA expression in the BLA. Treatment with SP alleviated both biochemical, molecular, and neuroanatomical deficits that induced by adolescent stress.

Conclusion

Our findings provide important evidence that SP as a non-pharmacological intervention during adolescent period can protect against chronic stress-induced neuroanatomical biochemical, and molecular deficits in adulthood, and thus, reduce stress-related disorders.

Keywords:

• adolescent stress
• amygdala
• BDNF
• dendritic remodeling
• Spirulina
• oxidative markers

Acknowledgments

The authors gratefully acknowledge the deputy of research and technology of Semnan University of Medical Sciences and the staff of the Research Center of Physiology for their support throughout the study. In addition, Mr. Nasroallah Moradi-Kor carried out this work in partial project fulfillment of the requirements to obtain the Ph.D. degree in Neuroscience.

Author Contributions

All authors contributed to data analysis, drafting and revising the article, gave final approval of the version to be published, and agree to be accountable for all aspects of the work.

Disclosure

The authors report no conflicts of interest in this work.

Additional information

Funding

This study was supported by grants from Semnan University of Medical Sciences and Iran National Science Foundation (#95836886).