The Neuroprotective Propensity of Organic Extracts of Acacia stenophylla Bark and Their Effectiveness Against Scopolamine-/Diazepam-Induced Amnesia in Mice

Abstract

Background

Alzheimer’s disease (AD) is a neurodegenerative disorder that is more prevalent in the elderly. There is extensive literature on using Acacia species against central nervous system disorders, although Acacia stenophylla has not been investigated for any neuroprotective potential. The purpose of the study was to elucidate the ameliorative effect of ethyl acetate (ASEE) and butanol (ASB) extracts from the bark of A. stenophylla on memory deficits and cognitive dysfunction in scopolamine- or diazepam-induced amnesia in mice.

Methods

The phytochemical constituents of the extracts of A. stenophylla were determined by GC-MS and the in vitro anticholinesterase plus antioxidant activities were also evaluated. Anti-amnesic effects were determined employing the open field test, elevated plus maze (EPM), Morris water maze (MWM), and Y-maze paradigms.

Results

The in vitro cholinesterase assays disclosed a concentration-dependent inhibition of both AChE and BuChE with IC₅₀ values of 28.48 and 44.86 µg/mL for the ASEE extract and 32.04 and 50.26 µg/mL for the ASB extract against AChE and BuChE respectively. DPPH and H₂O₂ antioxidant assays revealed respective IC₅₀ values for the ASEE extract of 28.04 and 59.84 µg/mL, plus 32.77 and 64.65 µg/mL for ASB extract. The findings revealed that both extracts possessed substantial antioxidant properties. Furthermore, these fractions restored scopolamine- and diazepam-induced memory deficits in a dose-dependent manner, as observed by a significant decrease in the transfer latency in EPM, reduction in escape latency, added time spent in the target quadrant in the MWM, and elevated spontaneous alternation behavior (SAB) in the Y-maze test.

Conclusion

The ameliorative effect of A. stenophylla on scopolamine- and diazepam-induced amnesia can be attributed to antioxidant and anticholinesterase activity. Consequently, the use of A. stenophylla might be exploited in the alleviation of oxidative stress and the management of AD.

Keywords:

• Acacia stenophylla
• amnesia
• oxidative stress
• cognitive deficits
• acetylcholinesterase

Data Sharing Statement

All the data has been either included in the manuscript.

Ethics Statement

Institutional Review Board Statement: The experimental procedures on animals were performed under the approval of Ethics Committee of the Department of Pharmacy, University of Peshawar (registration number: 12/EC-17/Pharm). The experimental procedures on animals were performed according to with the relevant guidelines and regulations of the United Kingdom Animals (Scientific procedures) Act 1986.

Acknowledgments

The authors thank the Department of Pharmacy, University of Peshawar, and the Department of Botany, Islamic college University Peshawar, for providing the facilities necessary to conduct this research. Yusuf S. Althobaiti was supported by Taif University Researchers Supporting Project number (TURSP-2020/78), Taif University, Taif, Saudi Arabia. Fahad S Alshehri would like to thank the ‎Deanship of Scientific Research at Umm Al-Qura University ‎for supporting this work by grant code (22UQU4310453DSR04).

Author Contributions

All authors made a significant contribution to the work reported, whether that is in the conception, study design, execution, acquisition of data, analysis and interpretation, or in all these areas; took part in drafting, revising or critically reviewing the article; gave final approval of the version to be published; have agreed on the journal to which the article has been submitted; and agree to be accountable for all aspects of the work.

Disclosure

All the authors declare no conflicts of interest in relation to this work.