Activity of Deposited Lysozyme on Contemporary Soft Contact Lenses Exposed to Differing Lens Care Systems

Abstract

Purpose

The amount of protein deposition on soft contact lenses and to what extent the proteins are denatured may have an impact on comfortable wearing times of contact lenses. The purpose of this study was to evaluate the effects of two lens care systems on total protein and the quantity and activity of lysozyme deposited on worn senofilcon A, silicone hydrogel contact lenses.

Participants and Methods

Thirty symptomatic soft contact lens wearers were enrolled into a 4-week prospective, randomized, bilateral eye, daily-wear, crossover, double-masked study. Participants were fitted with biweekly senofilcon A lenses and were assigned either a polyquaternium-1 and myristamidopropyl dimethylamine-containing system (OPTI-FREE RepleniSH) or a peroxide-based system (CLEAR CARE). After each wear period, proteins were extracted from the lenses and analyzed for total protein, total lysozyme quantity and activity.

Results

The use of either the peroxide-based system or the polyquaternium-1 and myristamidopropyl dimethylamine-containing system resulted in no difference (P>0.05) to the amount of total protein deposited on the lenses (6.7 ± 2.8 micrograms/lens versus 7.3 ± 2.8 micrograms/lens, respectively) or to the amount of denatured lysozyme deposits (0.8 ± 0.7 versus 0.9 ± 0.7 micrograms/lens), respectively. The total amount of lysozyme deposited on the lenses was significantly lower when using the peroxide-based system (1.3 ± 0.9 micrograms/lens) compared to the polyquaternium-1 and myristamidopropyl dimethylamine-containing system (1.7 ± 1.0 micrograms/lens) (P=0.02).

Conclusion

The inactivation of lysozyme deposited on senofilcon A lenses when disinfected with the peroxide-based or the polyquaternium-1 and myristamidopropyl dimethylamine-containing systems were neither statistically nor clinically significant and the overall amounts of denatured lysozyme recovered from the lenses were low (<1 microgram/lens).

Keywords:

• contact lens
• contact lens care system
• lysozyme
• protein activity
• silicone hydrogel

Data Sharing Statement

The individual participant clinical data that support the findings of this study are not available for review.

Consent for Publication

Participants have consented for the submission of results of the study for publication.

Acknowledgments

Over the past 3 years CORE has received research support or lectureship honoraria from Alcon, Allergan, Allied Innovations, Aurinia Pharma, BHVI, CooperVision, GL Chemtec, iMedPharma, J&J Vision, Lubris, Menicon, Nature’s Way, Novartis, Ophtecs, Ote Pharma, PS Therapy, Santen, Shire, SightGlass, SightSage and Visioneering.

Disclosure

 L Jones is a consultant and/or serves on advisory boards for Alcon, CooperVision, Johnson & Johnson Vision, Novartis and Ophtecs.  L Jones also reports grants from Alcon during the conduct of the study. His research group has contracts and/or receives personal fees from Alcon, Allergan, Allied Innovations, Aurinia Pharma, BHVI, CooperVision, GL Chemtech, i-Med Pharma, Johnson & Johnson Vision, Lubris, Menicon, Natures Way, Novartis, Ophtecs, Ote Pharma, PS Therapy, Santen, Shire, SightGlass, SightSage, and Visioneering, outside the submitted work. L Subbaraman and A Ng were employed at CORE while the study was conducted. A Ng is currently employed at Market Lane Optical, Woodbridge, Ontario, Canada and L Subbaraman is currently employed at Alcon Research, LLC, Vision Care Franchise, Johns Creek, Georgia, USA. The authors report no other conflicts of interest in this work.

Additional information

Funding

This study was funded by Alcon Research, LLC, USA.