Ocular Findings in Patients with COVID-19: Impact on Eye Banking [Letter]

Dear editor

With great interest, we have read the article by Qu et al on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission through the ocular route.¹ We would like to discuss the triggered impact on eye banking and corneal transplantation.

The authors reported a high potential of SARS-CoV-2 contagion of the ocular surface through hand-eye contact and aerosols and a possible transfer to other systems via nasolacrimal route or blood metastasis.¹ No mention has been made of possible SARS-CoV-2 contagion through ocular tissue transplantation, such as corneal transplantation, which may constitute another form of “ocular route”.

This question, which is particularly concerning for eye banks, was already investigated by Casagrande et al. They reported RNA traces in 6 out of 11 (55%) corneas of deceased donors with SARS-CoV-2 activity (viremia), or recent exposure. Nevertheless, no presence of viral structural protein could be confirmed in any corneal tissue.² Another study conducted by Bayyoud et al on 10 bulbi could not detect any signs of SARS-CoV-2 in neither conjunctiva, nor anterior chamber fluid nor corneal tissues of infected donors.³ These findings suggest that, even in donors with pre-mortem acute SARS-CoV-2 infection, the rate of contaminated ocular tissues post-mortem is very low and without any strong evidence of viral replicability. Therefore, the risk for SARS-CoV-2 contagion via corneal transplantation through the “ocular route” is minor.

Being part of university corneal transplantation centers with on-site eye bank, we have routinely pursued explanting donor corneas during the pandemic, excluding SARS-CoV-2 positive or coronavirus disease 2019 (COVID-19) suspicious donors in accordance to European Eye Bank Association (EEBA) and Global Alliance of Eye Bank Associations (GAEBA) recommendations. While most of our donors have been tested with reverse-transcriptase-polymerase-chain-reaction (RT-PCR) for SARS-CoV-2 pre-mortem during the inpatient stay (and were confirmed “negative”), a substantial proportion of donors resulted from the cooperation with our Institute for Anatomy (body donors) or with external institutions and had an unknown SARS-CoV-2 status. None of the post-mortem RT-PCR of the nasopharyngeal (0/199) and conjunctival swabs of these donors (0/262), performed – after flushing the ocular surface with 5% povidone-iodine for 5 minutes – in conjunction with the excision of 15 mm corneoscleral buttons from all post-mortem donors since April 2020, revealed any presence of SARS-CoV-2. We therefore conclude that the risk of SARS-CoV-2 contamination via corneal transplantation, after exclusion of infected or highly suspicious donors, is very low to absent.

In 2020, many eye banks drastically reduced or even ceased their activity as a preventive measure during the pandemic.⁴ Facing the lack of evidence for post-mortem SARS-CoV-2 activity or replicability in ocular tissues, and considering the increasing cumulative number of patients needing a corneal tissue for transplantation, we recommend to continue collection of all available corneas, as long as the appropriate precautions are taken. Considering the possibility of ocular transmission through “ocular route”, only tissue from donors with confirmed SARS-CoV-2 infection or with a high clinical suspicion for COVID-19 should not be selected for transplantation purposes.⁵

Disclosure

The authors report no conflicts of interest in this communication.