Characteristics of Corneal Subbasal Nerves in Different Age Groups: An in vivo Confocal Microscopic Analysis

Abstract

Purpose

To determine the normative characteristics of corneal subbasal nerves in different age groups using laser scanning in vivo confocal microscopy (IVCM).

Patients and Methods

This descriptive observational study recruited healthy subjects (aged 20–60 years) from Siriraj Health-Screening Center. Excluded were individuals who had abnormal ocular symptoms, previous ocular surgery, a history of any diseases related to systemic and/or corneal neuropathy, or abnormal corneal sensitivity. Corneal IVCM (HRT3/Rostock Corneal Module) was performed at the central cornea to analyze the subbasal nerve plexus. The corneal nerve characteristics, comprising the number and density of nerves (main nerve trunks, branches, and total nerves) were analyzed using the NeuronJ program, and the corneal nerve tortuosity was graded. The correlations between the subbasal nerve density, tortuosity and age were then analyzed.

Results

Eighty subjects were enrolled, with twenty in each of four age groups (20–30, >30–40, >40–50, and >50–60 years). Overall, the mean number and density of main nerve trunks were 27.93±0.81/mm² and 11.22±0.30 mm/mm², respectively. As of the nerve branches, the average number and density were 103.56±2.37/mm² and 9.15±0.30 mm/mm², respectively. The total nerve density was 20.37±0.39 mm/mm². There were no significant differences between subbasal nerve parameters of the four age groups. It is noteworthy that 65% of the subjects aged over 40 years revealed high-grade nerve tortuosity.

Conclusion

The corneal subbasal nerve numbers and densities were not significantly different among a healthy population aged 20–60 years. However, there was a trend towards high tortuosity of the corneal nerve in people aged over 40 years.

Keywords:

• in vivo confocal microscopy
• IVCM
• corneal subbasal nerves
• normal characteristics
• age

Disclosure

The authors report no conflicts of interest and have no proprietary interest in any of the materials mentioned in this article.

Additional information

Funding

This study is supported by the Research Development Fund of the Faculty of Medicine Siriraj Hospital, Mahidol University (No. R016031027). The funding organization has no role in the design or conduct of this study.