Gene transfection in primary stem-like cells of giant cell tumor of bone

Abstract

The neoplastic stem-like stromal cell of giant cell tumor of bone (GCT) survives for multiple passages in primary culture with a stable phenotype, and exhibits multipotent characteristics. The pathophysiology of this tumor has been studied through the primary culture of these cells. However, successful gene transfer of these cells has not been reported to date. In this short report, we describe the development of the first reported technique that results in efficient gene transfection in primary stem-like cells of GCT.

Keywords:

• gene
• transfection
• primary cells
• TWIST
• giant cell tumor

View correction statement:

Gene transfection in primary stem-like cells of giant cell tumor of bone

Disclosure

The authors report no conflicts of interest in this work.