Identification of Liver Cancer Stem Cell Stemness Markers Using a Comparative Analysis of Public Data Sets

Abstract

Purpose

Comparative reanalysis of single-cell transcriptomics data to gain useful novel insights into cancer stem cells (CSCs), which are a rare subset of cells within tumors, characterized by their capability to self-renew and differentiate, and their role in tumorigenicity.

Patients and Methods

This project utilized publically available liver single-cell RNA-seq datasets of liver cancer and liver progenitor cell types to demonstrate how shared large amounts of data can generate new and valuable information. The data were analyzed using EdgeR differential expression analysis, with focus on a set of 34 known stemness markers.

Results

We showed that the expression of stemness markers SOX9, KRT19, KRT7, and CD24, and Yamanaka factors Oct4 and SOX2 in CSCs was significantly elevated relative to progenitor cell types, potentially explaining their increased differentiation and replication potential.

Conclusion

These results help to further document the complementary expression changes that give CSCs their distinct phenotypic profile. Our findings have potential significance to advance our knowledge of the important genes relevant to CSCs.

Keywords:

• Yamanaka factors
• single cell sequencing
• CSCs
• RNA-seq; cancer

Acknowledgments

This work was in part funded by NIH grant UL1TR001433.

Disclosure

The authors report no conflicts of interest in this work.