Management of traumatic tympanic membrane perforation: a comparative study

Abstract

This prospective study was conducted to evaluate the efficacy of sea buckthorn oil patches in treating traumatic tympanic membrane (TM) perforations. We enrolled 370 patients with traumatic TM perforations of different sizes. These patients were randomly assigned to control group and treatment group. In the treatment group, a sterile cotton patch with sea buckthorn oil was used to cover the TM perforations. In the control group, patients were treated with a sterile cotton patch. The healing rate and time were compared between the two groups. We found that the overall healing rate was significantly higher in the treatment group than in the control group. For middle and large TM perforations, sea buckthorn oil treatment led to a significant increase in the healing rate. At 2 months after injury, the duration of healing was, generally, shorter in the treatment group than in the control group (P<0.05). In conclusion, sea buckthorn oil patches are effective in treating middle and large TM perforations, which results in increased healing rates and decreased healing time.

Keywords:

• sea buckthorn oil
• tympanic membrane
• perforation
• regeneration

Introduction

Traumatic tympanic membrane (TM) perforation is an injury of the eardrum, which is frequently faced by otolaryngologists. Common causes to traumatic TM perforation include rapid change in ear pressure (eg, occurring when flying and scuba diving), thermal or chemical burns, direct penetrating trauma, and barotrauma.^1,2 The incidence of perforations of the TM due to trauma is on the increase consequent to trauma, and increased violence and accidents seen in present-day life.³ Ear buzzing, earache, and hearing loss are the major symptoms of TM perforation. In addition, TM perforation can increase the risk for middle ear infection or otitis media.⁴ Although most small perforations of the eardrum can be spontaneously healed, large TM perforations should be treated with myringoplasty.⁵

Sea buckthorn oil, which is extracted from the berries and seeds of the sea buckthorn plant, contains abundant vitamins, amino acids, and essential trace elements.⁶ A previous study has shown that sea buckthorn oil can be used to increase the healing rate of TM perforations.⁷ In the present study, we enrolled 370 patients with traumatic TM perforation and evaluated the therapeutic efficacy and safety of sea buckthorn oil in the treatment of different sizes of TM perforations.

Materials and methods

Patients

This study was approved by the Ethics Review Board of the Second Affiliated Hospital of Xi’an Jiaotong University (2009–26). We enrolled 370 patients with traumatic TM perforation who were treated at our hospital between January 2010 and March 2015. There were 196 males and 174 females, with the mean age of 32.3±13.1 years (range, 6–57 years). Overall, 226 cases presented a perforation in the left ear and 144 in the right ear. Triangle perforation was detected in 96 cases, spindle perforation in 83 cases, oval perforation in 62 cases, slit-shaped perforation in 78 cases, and irregular perforation in 51 cases. Inclusion criteria were history of traumatic injury, ear discomfort after injury, perforation of the eardrum, absence of infection, and no local treatment. Exclusion criteria were presence of otitis media, injury of auditory ossicles, allergic to sea buckthorn oil, and refusal to treatment and follow-up. Each patient signed the informed consent. Patients were prospectively and randomly assigned to control group (n=183) and treatment group (n=187). Treatment group was divided according to the size of perforation: small perforation, middle perforation, and large perforation.

Classification criteria for perforation

The classification was according to the size of perforation: small perforation, <1 quadrants or maximum diameter <3 mm; middle perforation, >1 quadrants and <2 quadrants or maximum diameter between 3 and 5 mm; and large perforation, >2 quadrants or maximum diameter >5 mm.

Treatment

In the treatment group, a sterile cotton patch with sea buckthorn oil (Shannxi Aikang Shaji Company, Xi’an, People’s Republic of China) was used to cover the TM perforation after iodophor disinfection of the outer ear canal. The patch was changed once every week. The control group was treated with routine observation without sea buckthorn oil. The external auditory canal of patients was disinfected with iodophor. The site of perforation was not treated and maintained dry ear, followed up once a week. Ear endoscope was used to observe the growth change of perforation between two groups. Meanwhile the two groups were to prevent infection, to maintain patency of the eustachian tube, to prevent a cold, and to prevent water. If there was a local infection, the treatment group was treated with oral antibiotics and without sea buckthorn oil for one time. The control group was given anti-infection treatment.

Follow-up

The healing of the TM perforation was monitored for 1 and 2 months. The healing rate was used as the evaluation index between the two groups. The average healing time and the healing area were observed and compared. Full healing was defined as complete closure of perforation, and partial healing as a reduction in perforation size. No change in perforation size was considered as treatment failure.

Statistical analysis

Statistical analysis was performed using SPSS 17.0 software (SPSS, Chicago, IL, USA). All data are presented as mean ± SD. Differences in mean healing time (satisfied normality and homogeneity of variances) were analyzed using Student’s t-test; the healing rate between two groups was using chi-square test (including the Pearson chi-square test and continuously corrected chi-square test). Subgroup data were analyzed by analysis of variance. A P-value of <0.05 was considered statistically significant.

Results

Demographics of patients

There was no significant difference about sex, age, cause of disease, and size of perforation between control group and treatment group (Table 1).

Table 1 Demographics and clinical characteristics between two groups

Group	Case no	Sex		Age (years), mean ± SD	Causes of disease		Size of perforation
		Male	Female		Direct damage	Indirect damage	Small	Middle	Large
Treatment	187	101 (54.0%)	86 (46.0%)	30.6±12.1	153 (81.8%)	34 (18.2%)	67 (35.8%)	62 (33.2%)	58 (31%)
Control	183	95 (51.9%)	88 (48.1%)	33.4±13.7	157 (85.8%)	26 (14.2%)	63 (34.4%)	59 (32.3%)	61 (33.3%)

Morphological features

The newly formed TM had a smooth surface with similar thickness to normal counterparts. TM scarring was less frequently detected in the treat group than in the control group (Figure 1).

Figure 1 The typical cases of all kinds of membrane perforation.

Notes: A representative case of tympanic membrane perforation (A1) before treatment of small perforation; (A2) after treatment of small perforation; (B1) before treatment of small perforation in control group; (B2) self-healing of small perforation in control group; (C1) before treatment of middle perforation; (C2) full healing treatment for two times with sea buckthorn oil; (D1) before treatment of large perforation; (D2) after treatment for three times with sea buckthorn oil; and (D3) full healing after treatment for five times with sea buckthorn oil.

Perforation healing rate

At 1 month after perforation, the overall healing rates were 61.0% and 45.0% in the treatment group and control group, respectively (P<0.01; Table 2). At 2 months after perforation, the overall healing rates of 71.6% and 54.1% were noted in the treatment group and the control group, respectively (P<0.01; Table 3). For middle and large TM perforations, the healing rates were 59.7% and 39.6% in the treatment group and 13.6% and 0 in the control group, respectively, at 1 month after perforation (P<0.05 for both comparisons). The differences in the healing rates for middle and large TM perforations remained significant between the two groups at 2 months after perforation (P<0.05). In contrast, the two groups did not differ significantly in the healing rates for small TM perforations (P>0.05).

Table 2 Comparison of healing rate between two groups for 1 month

Group	Cases no	Full healing	Partial healing	Failure
Treatment
Small	67	54 (80.6%)	10 (15%)	3 (4.4%)
Middle	62	37 (59.7%)	21 (33.9%)	4 (6.4%)
Large	58	23 (39.6%)	32 (55.2%)	3 (5.2%)
Control
Small	63	45 (71.4%)	14 (22.2%)	4 (6.4%)
Middle	59	8 (13.6%)	27 (45.8%)	34 (57.6%)
Large	61	0	16 (26.2%)	45 (73.8%)

Table 3 Comparison of healing rate between two groups for 2 months

Group	Cases number	Full healing	Partial healing	Failure
Treatment
Small	67	62 (92.5%)	2 (3%)	3 (4.5%)
Middle	62	50 (80.6%)	8 (12.9%)	4 (6.5%)
Large	58	38 (65.5%)	12 (20.7%)	8 (13.8%)
Control
Small	63	59 (93.7%)	0	4 (6.3%)
Middle	59	32 (54.2%)	3 (5.1%)	24 (40.7%)
Large	61	8 (13.1%)	11 (18%)	42 (68.9%)

Duration of perforation full healing

At 1 month after injury, treatment with sea buckthorn oil significantly reduced the time to full healing for small TM perforations, compared to the control group (P<0.05; Table 4). However, with regard to middle and large TM perforations, the duration of healing did not differ significantly between the treatment and control groups (P>0.05). At 2 months after injury, the duration of healing was generally shorter in the treatment group than in the control group, irrespective of the size of TM perforations (P<0.05).

Table 4 Comparison of duration of perforation healing between two groups (days)

Observation time	Number of healing	Small	Middle	Large
Treatment
1 month	114	13.24±7.12	19.63±8.12	29.38±11.22
2 months	150	16.45±9.67	26.79±10.36	29.97±10.89
Control
1 month	53	19.91±6.54	24.39±9.51	–
2 months	99	26.17±13.31	38.76±13.52	47.97±9.85

Note: Data presented as mean ± standard deviation.

Discussion

Traumatic TM perforation is a common clinical condition and most of them heal spontaneously within 4 weeks.⁸ Surgical treatment is indicated if TM perforations are symptomatic and large. A prospective, nonrandom study by Sayin et al demonstrated that there was a spontaneous healing rate of 85.6% among 97 patients with TM perforations (59.8% small, 36.1% middle, and 4.1% large perforations).⁹ In this study, we showed that the differences between the overall healing and spontaneous healing rates were statistically significant in the control group. The spontaneous healing rate rises with time, but decreases with the increase in the perforation size. The higher spontaneous healing rate observed in previous studies may be due to a smaller percentage of large perforations and prolonged duration. When the waiting time for spontaneous perforation healing is long, the risk for otitis media was increased.¹⁰ In addition, there is an evidence that the site of perforation and blood supply also affect the spontaneous healing of TM perforations.¹¹ Therefore, it is recommended that large TM perforations need active therapy.

Many efforts have been made to develop drug-loaded patches for repairing TM perforations. For instance, gelatin sponge, basic fibroblast growth factor, and sea buckthorn oil have shown the ability to facilitate the healing of TM perforations.^12–14 These agents can promote epithelial cell proliferation and cell–cell connection, consequently accelerating the repair of perforations and improving hearing. It is known that sea buckthorn oil is rich in vitamins, amino acids, and essential trace elements. The content of vitamin C is 35-fold greater in sea buckthorn oil than in oranges.¹⁵ Sea buckthorn oil has a high level of zinc (2.11 pm relative to the serum level of 0.8–1.2 ppm in humans). It has been reported that zinc can enhance the healing of traumatic injury.¹⁶ Compelling evidence indicates that sea buckthorn oil has the ability to promote tissue regeneration and no toxicity to the ear.^17,18 Zhang and Fan reported that treatment with sea buckthorn oil remarkably enhances the proliferation of TM epithelial stem cells and their migration to lesions, which are coupled with increased amounts of mitochondria and thickening of the rough endoplasmic reticulum.¹⁹ Other researchers found that flavonoids of sea buckthorn oil can induce blood vessel dilatation and aid in the recovery of middle ear function.²⁰

In this prospective study, we compared the healing of TM perforations in the sea buckthorn oil and control groups. We found that the overall healing rate differed significantly between the two groups at different time points. The application of sea buckthorn oil patches promoted the healing of TM perforations. Compared to the control group, the duration of healing was significantly shorter in sea buckthorn oil group. Therefore, our data indicate that sea buckthorn oil patches are beneficial in the repair of TM perforations, especially middle and large perforations, which can increase healing incidences and reduce healing duration.

It is estimated that repair of TM perforations using patches can lead to a healing rate of 80%–95%, which is comparable to that achieved by myringoplasty.²¹ The patch-based treatment strategy has a cost-effective advantage and does not need autologous tissues.²² The regenerated TM by myringoplasty has a different structure than that of normal TM, which hampers the improvement of patient hearing.²³

Conclusion

Sea buckthorn oil patches are beneficial in repairing middle and large TM perforations, increasing healing rates and shortening the healing time. This treatment strategy has a cost-effective and low-risk advantage over surgical approaches. Meanwhile, it can save the medical expenses and is worthy of clinical use.

Disclosure

The authors report no conflicts of interest in this work.