Methods, potentials, and limitations of gene delivery to regenerate central nervous system cells

Abstract

This review evaluates methods, success and limitations of transgenes delivery in central nervous system (CNS). Both viral and nonviral (such as liposome mediated) methods, expression and stability of transgenes have been discussed. The controlled expression and delivery techniques of transgene at the injured or diseased sites have also been discussed. Mifepristone (RU486) and tetracycline-based switch system for controlled expression could be a very useful tool for clinical purposes. Here we emphasized the importance and consequences of viral- and nonviral-mediated transgenes transfer and therapeutic ability along with advantages of controlled expressions.

Keywords:

• transgenes
• viral vector
• nonviral vector
• RU486
• tetracycline
• CNS

Acknowledgments

Authors acknowledge financial support from the Canadian Institute of Health Research (CIHR) grant to Prakash and University Grants Commission (UGC), India to AK, TDS, and SKS. We would also like to thank technical support from Jasmine Bhathena.