Marked differences in fatty acid profiles of some planktonic and benthic marine dinoflagellates from Malaysian waters

Abstract

G. Usup, S.Z. Hamid, P.K. Chiet, C.K. Wah and A. Ahmad. 2007. Marked differences in fatty acid profiles of some planktonic and benthic marine dinoflagellates from Malaysian waters. Phycologia 47: 105–111. DOI: 10.2216/07-55.1

This study was carried out to characterize the fatty acid profiles of some planktonic and benthic marine dinoflagellates from Malaysian waters. Clonal batch cultures of Alexandrium affine, A. leei, A. minutm, A. tamarense, A. tamiyavanichii, Coolia monotis, Prorocentrum emarginatum, P. mexicanum, Ostreopsis ovata and Amphidinium sp. were harvested at late exponential phase, and total lipid was extracted. Samples were derivatized to produce fatty acid methyl esters (FAMEs). FAMEs were analyzed on a gas chromatograph with flame ionization detection. The total number of fatty acids detected in the clones ranged from 10 in the A. tamarense AtPA04 clone to 22 in the C. monotis CmPL01 clone. Fatty acids found in all clones were myristic acid (14 : 0), palmitic acid (16 : 0), stearic acid (18 : 0), linoleic acid (18 : 2ω6c) and oleic acid (18 : 1ω9c). In all clones only a few fatty acids were dominant. In the Alexandrium clones the dominant fatty acids were 16 : 0, 18 : 0, cis-13,16-docosadienoic acid (22 : 2), 18 : 2ω6c and 18 : 1ω9c. There was almost complete absence of omega-3 polyunsaturated fatty acids (PUFA) in the Alexandrium clones. In the benthic species the major fatty acids were 16 : 0, eicosapentaenoic acid (EPA, 20 : 5ω3), docosahexaenoic acid (22 : 6ω3), 18 : 2ω6c and 18 : 1ω9c. In the Prorocentrum clones the major fatty acids were 14 : 0, 16 : 0, palmitoleic acid (16 : 1) and EPA. Total PUFA content in the benthic species were 37%–56%, while in the planktonic species the content was 19%–44%. The fatty acid profiles could not differentiate between species. However, cluster analysis and principal components analysis were able to clearly discriminate between the Alexandrium group, Prorocentrum group and benthic species group.

ACKNOWLEDGMENTS

This study was funded by the Malaysia government through research grants IRPA 09-02-02-EA0079 and 02-01-02-SF0203.