Nitrate reduces the negative effect of UV radiation on photosynthesis and pigmentation in Gracilaria tenuistipitata (Rhodophyta): the photoprotection role of mycosporine-like amino acids

Abstract

Barufi J.B., Mata M.T., Oliveira M.C. and Figueroa F.L. 2012. Nitrate reduces the negative effect of UV radiation on photosynthesis and pigmentation in Gracilaria tenuistipitata (Rhodophyta): the photoprotection role of mycosporine-like amino acids. Phycologia 51: 636–648. DOI: 10.2216/10.77.1

Photoprotection of the agarophyte red alga Gracilaria tenuistipitata against ultraviolet radiation (UVR) was investigated in algae submitted for 1 week to photosynthetically active radiation (PAR, 260 μmol photons m⁻² s⁻¹) or PAR + UVR (UV-A, 8.13 W m⁻² and UV-B, 0.42 W m⁻²) under different nitrogen concentrations: 0, 0.1, and 0.5 mM of NO₃⁻. Photosynthetic pigments decreased during the time of the experiment mainly under low nitrogen supply and UVR. Incubation under high nitrogen supply (0.5 mM) sustained the photosynthetic levels over time. In contrast, mycosporine-like amino acids (MAAs) increased up to eightfold in the presence of UVR and 0.5 mM NO₃⁻. Under PAR + UVR, maximal quantum yield was positively correlated to MAA abundance, whereas under PAR no correlation was found. The photosynthetic yield of algae cultivated during seven days under PAR + UVR was less affected by a 30-min exposure of high UVR (16 W m⁻²) and fully recovered after transferring to low PAR irradiances, whereas algae kept under PAR were more affected by UV exposure and no full recovery was observed. Growth rates decreased after three days in the presence of UVR and under low nitrate supply. However, these rates were similar when compared with treatments of PAR and PAR + UVR after seven days, with the exception of samples in 0 mM NO₃⁻, indicating that the acclimation after one week's exposure is related to nitrate supply. In conclusion, the lowest negative effect of UVR on photosynthesis and growth rate in high N-supply-grown algae could be explained by the stimulation of photoprotection mechanisms, such as accumulation of MAAs. Photostimulation of MAA accumulation by UVR under high N supply was observed in G. tenuistipitata even after 20 years in culture without the induction of this photomorphogenic light signal.

Key Words::

• Chlorophyll fluorescence
• Gracilaria tenuistipitata
• Mycosporine-like amino acids
• Nitrogen supply
• UV radiation

ACKNOWLEDGEMENTS

Financial support was provided by the Ministry of Science and Technology of Spain (Project Ecolife, CGL2008 05407-C03-01) and Junta de Andalucía (RNM-295). J.B.B. thanks the grant from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and M.C.O. thanks Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) from the Ministry of Education of Brazil and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP).