Molecular identification of gelidioid algae (Gelidiales, Rhodophyta) from Singapore with a description of Gelidium sentosaense sp. nov.

Abstract:

Gelidioid specimens from Singapore were analyzed using sequences of cox1 and rbcL to identify the species. Both datasets revealed the presence of four species: Gelidiella acerosa, Pterocladiella bartlettii, Pterocladiella caerulescens and Gelidium sentosaense sp. nov., described here. Gelidium sentosaense formed small tufts up to 1 cm high and occurred in monospecific patches on cement beds at the outlet of drainpipes at intertidal areas. A morphological investigation of fresh specimens collected from Singapore revealed that G. sentosaense was similar to Gelidium pusillum, which is limited to North Atlantic coasts. However, it was distinguished by longitudinal striations present on the surface of branches and rhizines scattered in the inner cortex and medulla. Phylogenetic analyses of rbcL and cox1 sequences revealed that G. sentosaense consistently formed a clade with Gelidium sp. from Oman and Gelidium pluma from Hawaii but it was distinct from previously described Gelidium species.

Key Words::

• cox1
• Gelidiaceae
• Gelidiella acerosa
• Pterocladiella
• rbcL
• Southeast Asia
• Taxonomy

ACKNOWLEDGEMENTS

We thank National Biodiversity Centre, National Parks Board, Singapore, for the research permit NP/RP12-193 for specimens collection, and Paul John L. Geraldino for collection of specimens in Singapore and field guidance during 19th International Seaweed Symposium in Bali, Jung Yeon Kim for sequencing and D. Wilson Freshwater for valuable comments and corrections. This work was supported by Marine Biotechnology program grants from the Ministry of Ocean Sciences and Fisheries to SMB.

SUPPLEMENTARY DATA

Supplementary data associated with this article can be found online at http://dx.doi.org/10.2216/15-133.1.s1.