Screening Circular RNA Related to Chemotherapeutic Resistance in Breast Cancer

Abstract

Aim: We aimed to identify circular RNAs (circRNAs) associated with breast cancer chemoresistance. Materials & methods: CircRNA microarray expression profiles were obtained from Adriamycin (ADM) resistant MCF-7 breast cancer cells (MCF-7/ADM) and parental MCF-7 cells and were validated using quantitative real-time reverse transcription PCR. The expression data were analyzed bioinformatically. Results: We detected 3093 circRNAs and identified 18 circRNAs that are differentially expressed between MCF-7/ADM and MCF-7 cells; after validating by quantitative real-time reverse transcription PCR, we predicted the possible miRNAs and potential target genes of the seven upregulated circRNAs using TargetScan and miRanda. The bioinformatics analysis revealed several target genes related to cancer-related signaling pathways. Additionally, we discovered a regulatory role of the circ_0006528-miR-7–5p-Raf1 axis in ADM-resistant breast cancer. Conclusion: These results revealed that circRNAs may play a role in breast cancer chemoresistance and that hsa_circ_0006528 might be a promising candidate for further functional analysis.

Keywords::

• adriamycin
• breast cancer
• chemoresistance
• circular RNA
• microarray

Financial & competing interests disclosure

This work was supported by grants from the National Natural Science Foundation of China (81472485), the Project of Six Talent Peaks of Jiangsu Province (WSW-014), and the Project of the Wuxi Health and Family Planning Commission (YGZXM14038). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.tandfonline.com/doi/suppl/10.2217/3dp-2022-0019

Additional information

Funding

This work was supported by grants from the National Natural Science Foundation of China (81472485), the Project of Six Talent Peaks of Jiangsu Province (WSW-014), and the Project of the Wuxi Health and Family Planning Commission (YGZXM14038). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.