Transcriptional Regulation on The Gene Expression Signature in Combined Allergic Rhinitis and Asthma Syndrome

Abstract

Aim: This study was intended to evaluate transcriptional regulation of gene expression signatures in combined allergic rhinitis and asthma syndrome (CARAS). Materials & methods: The blood samples of three patients with CARAS, three patients with allergic rhinitis and three normal controls were obtained. The cuffdiff, miRDeep2 and DEGseq were used to quantify the expression of genes and miRNAs, respectively. And p-value < 0.01 and false discovery rate < 0.001 were considered as significant differences of genes and miRNAs, respectively. Gene ontology and the Kyoto Encyclopedia of Genes and Genomes were used to analyze the biological function. And the cut-off value for significance was p < 0.05. Results:SLC14A1, SNCA, TNS1, KAT2B and PARP1 were regulated by hsa-miR-93–5p, hsa-miR-92a-3p and hsa-miR-21–5p. Additionally, phagosome (p = 0.00627769839083361) was the only significantly enriched signal pathway involving HLA-DOA, TUBB2A and MRC2. Conclusion: Disordered expression of genes under the regulation of miRNAs may play an important role in CARAS.

Keywords::

• combined allergic rhinitis and asthma syndrome
• differentially expressed genes
• differentially expressed miRNAs
• phagosome signal pathway

Financial & competing interests disclosure

This study was supported by the National Natural Science Foundation of China (81270077), Jiangsu province “six big talent peak” project (WSN092), Changzhou High-Level Medical Talents Training Project (2016CZLJ017), Jiangsu province “333 talents” project (BRA2016119) and the Changzhou Sci & Tech Program (CJ20179031). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

Additional information

Funding

This study was supported by the National Natural Science Foundation of China (81270077), Jiangsu province “six big talent peak” project (WSN092), Changzhou High-Level Medical Talents Training Project (2016CZLJ017), Jiangsu province “333 talents” project (BRA2016119) and the Changzhou Sci & Tech Program (CJ20179031). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.