Screening Circular RNA Related to Chemotherapeutic Resistance in Osteosarcoma by RNA Sequencing

Abstract

Aim: To identify circular RNAs (circRNAs) related to osteosarcoma (OS) chemoresistance. Materials & methods: CircRNA expression profile was performed in three paired human chemoresistant and chemosensitive OS cell lines by next-generation sequencing. Quantitative real-time-PCR (qRT-PCR) was used to confirm next-generation sequencing data. Bioinformatics analysis was conducted to predict their functions. Results: Eighty circRNAs were dysregulated in the chemoresistant OS cells compared with the control, after validated by qRT-PCR. Bioinformatics analysis showed that some pathways related to drug metabolism were significantly enriched. Additionally, hsa_circ_0004674 was distinctly increased in OS chemoresistant cells and tissues, related to poor prognosis. CircRNA-miRNA-mRNA pathways related to hsa_circ_0004674 were constructed by TargetScan and miRanda. Conclusion: CircRNAs may play a role in OS chemoresistance and hsa_circ_0004674 might be a candidate target.

Keywords::

• chemoresistance
• circRNA
• osteosarcoma

Financial & competing interests disclosure

This project was supported by the grants from the National Natural Science Foundation of China (No. 81572630), the Fundamental Research Funds for the Central Universities (No. 22120170216), Shanghai Pujiang Program of Shanghai Science and Technology Commission (No. 13PJD023) and Shanghai Jiaotong University Medical-Engineering Cross Research Fund (No. YG2012MS49). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

Additional information

Funding

This project was supported by the grants from the National Natural Science Foundation of China (No. 81572630), the Fundamental Research Funds for the Central Universities (No. 22120170216), Shanghai Pujiang Program of Shanghai Science and Technology Commission (No. 13PJD023) and Shanghai Jiaotong University Medical-Engineering Cross Research Fund (No. YG2012MS49). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.