Circular RNA CircVAPA Regulates Breast Cancer Cell Migration and Invasion Via Sponging miR-130a-5p

Abstract

Aim: We aimed to explore the roles of circular RNA, circVAPA in regulating cell migration and invasion of breast cancer. Materials & methods: CircVAPA expression was detected in breast cancer tissues and cells. The role of circVAPA was evaluated by MTT assay, wound-healing and transwell assay. The relationship between circVAPA and miR-130a-5p and the location of circVAPA were explored. Results: We discovered that circVAPA was dysregulated in breast cancer tissues and cells. Ectopic circVAPA regulated breast cancer migration, invasion and proliferation. CircVAPA was mainly expressed in the cytoplasm and could act as a miRNA sponge for miR-130a-5p, but did not regulate its parental gene. Conclusion: CircVAPA may promote migration and invasion capacity of breast cancer via harboring miR-130a-5p.

Keywords::

• breast cancer
• ceRNA
• circRNAs
• invasion
• metastasis
• migration
• miRNA
• proliferation
• survival
• VAPA

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.tandfonline.com/doi/suppl/10.2217/epi-2019-0124

Financial & competing interests disclosure

This research was supported by the National Key Research and Development Program of China (Grant Number 2016YFC0905900), National Natural Science Foundation of China (grant number 81872365), the ‘333’ Talent Project of Jiangsu Province (grant number 4 [2016]) and Innovation Team Construction Project of Nanjing Medical University (grant number JX102GSP201727), National Natural Science Foundation of China (grant number 81602551), Postgraduate Research and Practice Innovation Program of Jiangsu Province (Grant Number KYCX19_1196). The authors have no other relevant affiliations or financialinvolvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Additional information

Funding

This research was supported by the National Key Research and Development Program of China (Grant Number 2016YFC0905900), National Natural Science Foundation of China (grant number 81872365), the ‘333’ Talent Project of Jiangsu Province (grant number 4 [2016]) and Innovation Team Construction Project of Nanjing Medical University (grant number JX102GSP201727), National Natural Science Foundation of China (grant number 81602551), Postgraduate Research and Practice Innovation Program of Jiangsu Province (Grant Number KYCX19_1196). The authors have no other relevant affiliations or financialinvolvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.