Abstract
Aim: To comprehensively understand microbiota-regulated lincRNA–miRNA–mRNA networks in psychiatric disorders. Materials & methods: Integrated analyses of lincRNAs, mRNAs and miRNAs, obtained by microarray analysis of hippocampus from specific pathogen-free, germ-free and colonized germ-free mice, were performed. Results: Expression of 139 mRNAs, seven miRNAs and one lincRNA was restored following colonization. The restored transcripts were mainly involved in CREB and Ras/MAPK signaling pathways. RNA transcription and post-transcriptional regulation were the primary perturbed functions. Finally, 12 lincRNAs, six miRNAs and 47 mRNAs were included in a lincRNA–miRNA–mRNA network, and lincRNA0926-miR-190a-5p-Celf4 interactions may play a pivotal role in this regulatory network. Conclusion: This study provides clues for understanding the molecular basis of gut microbiota–brain interactions in depressive- and anxiety-like behaviors.
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Supplementary data
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Financial & competing interests disclosure
This study was supported by the National Key R & D Program of China (grant no. 2017YFA0505700), the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences (grant no. 2019PT320002) and the Natural Science Foundation Project of China (grant no. 81820108015). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript.
Funded writing assistance was utilized in the production of this manuscript, and the sources of funding for such assistance was the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences (grant no. 2019PT320002).
Ethical conduct of research
The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations.