hsa_circ_0001947 Suppresses Acute Myeloid Leukemia Progression Via Targeting hsa-miR-329-5p/CREBRF axis

Abstract

Aim: Accumulating evidence has indicated that circular RNAs (circRNAs) are involved in cancer biology. However, their roles in acute myeloid leukemia (AML) remain unclear. Therefore, we aimed to define novel circRNAs involved the development and progression of AML. Materials & methods: We used circRNAs microarray to determine the differential expression profile. Quantitative reverse transcription PCR analyzed the expression of hsa_circ_0001947. The siRNA assesses the function of hsa_circ_0001947in vitro and in vivo. A dual-luciferase and mimics/inhibitor were to determine the target gene relationship. Results:hsa_circ_0001947 functions as a tumor inhibitor to suppress AML cell proliferation through hsa-miR-329-5p/CREBRF axis. Conclusion:hsa_circ_0001947 may be as a novel potential biomarker for the treatment of AML.

Keywords::

• AML
• biomarker
• circRNAs
• CREBRF
• hsa_circ_0001947
• hsa-miR-329-5p
• therapy

Acknowledgments

The authors sincerely thank all the participants in the study.

Financial & competing interest disclosure

This work was supported by grants from National Natural Science Foundation of China (no. 91642110) and Shandong Provincial Natural Science Foundation (no. ZR2018PH012). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations.

Data sharing statement

All data generated or analyzed during this study are included in this published article and its supplementary information file.

Additional information

Funding

This work was supported by grants from National Natural Science Foundation of China (no. 91642110) and Shandong Provincial Natural Science Foundation (no. ZR2018PH012). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.No writing assistance was utilized in the production of this manuscript.