Dynamic Analysis of m6A Methylation Spectroscopy During Progression and Reversal of Hepatic Fibrosis

Abstract

Aim: To dynamically analyze the differential m6A methylation during the progression and reversal of hepatic fibrosis. Materials & methods: We induced hepatic fibrosis in C57/BL6 mice by intraperitoneal injection of CCl4. The reversal model of hepatic fibrosis was established by stopping drug after continuous injection of CCl4. Dynamic m6A methylation was evaluated using MeRIP-Seq in the progression and reversal of hepatic fibrosis at different stages. Result: During the hepatic fibrosis, differential m6A methylation was mainly enriched in processes associated with oxidative stress and cytochrome metabolism, while differential m6A methylation was mainly enriched in processes associated with immune response and apoptosis in the hepatic fibrosis reversal. Conclusion: m6A methylation plays an important role in the progression and reversal of hepatic fibrosis.

Keywords::

• differentially methylated genes
• hepatic fibrosis progression
• hepatic fibrosis reversal
• N6-methyladenosine

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.tandfonline.com/doi/suppl/10.2217/epi-2019-0365

Acknowledgments

We thank Cloudseq Biotech, Inc. (Shanghai, China) for the m6A-meRIP sequencing service and bioinformatics support.

Financial & competing interest disclosure

This work was mainly supported by the National Natural Science Foundation of China (81608129/81772941); Shanghai Municipal Health Bureau (2019cxjq03); and Shanghai Municipal Health Planning Commission Research Fund (201840261). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

Writing assistance was utilized in the production of this manuscript. This writing assistance was provided by Liwen Editing and funded by the National Natural Science Foundation of China (81608129/81772941).

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations.

Additional information

Funding

This work was mainly supported by the National Natural Science Foundation of China (81608129/81772941); Shanghai Municipal Health Bureau (2019cxjq03); and Shanghai Municipal Health Planning Commission Research Fund (201840261). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. Writing assistance was utilized in the production of this manuscript. This writing assistance was provided by Liwen Editing and funded by the National Natural Science Foundation of China (81608129/81772941).