Knockdown of Long Noncoding RNA AC245100.4 Inhibits the Tumorigenesis of Prostate Cancer Cells Via the STAT3/NR4A3 Axis

Abstract

Aim: To explore the role and mechanism of long noncoding RNA AC245100.4 and NR4A3 in prostate cancer (PCa). Methods: RNA-sequencing analysis was used to detect the downstream genes of AC245100.4. A series of gain- and loss-of-function approaches were used to investigate the roles of AC245100.4 and NR4A3. RNA immunoprecipitation was performed to examine the interaction between AC245100.4 and STAT3. Results: AC245100.4 was significantly upregulated in PCa cells and tissues. Knockdown of AC21500.4 significantly inhibited the tumorigenesis of PCa cells. Mechanistically, AC245100.4 deregulated the transcription of NR4A3 via increasing p-STAT3, which acted as a transcriptional repressor of NR4A3. Conclusion: Knockdown of long noncoding RNA AC245100.4 inhibits the tumorigenesis of PCa cells via the STAT3/NR4A3 axis.

Lay abstract

Long noncoding RNA has recently gained attention for the vital role it plays in the mechanism of prostate cancer (PCa). In this study, the authors found that long noncoding RNA AC245100.4 inhibited the tumor formation of PCa cells via the STAT3/NR4A3 axis. A deeper understanding of the specific mechanism of AC245100.4 in PCa tumor formation will provide insights into diagnostic and prognostic strategies for PCa.

Keywords::

• AC245100.4
• apoptosis
• NR4A3
• proliferation
• prostate cancer
• STAT3

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.tandfonline.com/doi/suppl/10.2217/fon-2021-0284

Author contributions

X Yu and J Zhao: conceptualization; W Yang and S Jiang: formal analysis; N Wang, R Li and X Yang: data curation; C Liu: writing the original draft; H Xie and P Lin: review and editing. All authors edited and approved the manuscript.

Financial & competing interests disclosure

This study was supported financially by the Fundamental Research Funds for the Provincial Universities (no. 2018-KYYWF-0432) and the Postdoctoral Scientific Research Developmental Fund of China (no. 212538). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

Writing assistance was provided by Freescience Editorial Tea.

Ethical conduct of research

The experimental protocol was established according to the ethical guidelines of the Helsinki Declaration and was approved by the Ethics Committee of Harbin Medical University.

Availability of data & material

All data generated or analyzed during this study are included in the published article.

Additional information

Funding

This study was supported financially by the Fundamental Research Funds for the Provincial Universities (no. 2018-KYYWF-0432) and the Postdoctoral Scientific Research Developmental Fund of China (no. 212538). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. Writing assistance was provided by Freescience Editorial Tea.