Identification of Histone Acetylation Modification Sites in the Striatum of Subchronically Manganese-Exposed Rats

Abstract

Aim: To explore the specific histone acetylation sites and oxidative stress-related genes that are associated with the pathogenesis of manganese toxicity. Methods: We employed liquid chromatography–tandem mass spectrometry and bioinformatics analysis to identify acetylated proteins in the striatum of subchronic manganese-intoxicated rats. Results: We identified a total of 12 differentially modified histone acetylation sites: H3K9ac, H3K14ac, H3K18ac, H3K56ac and H3K79ac were upregulated and H3K27ac, H3K36ac, H4K91ac, H4K79ac, H4K31ac, H2BK16ac and H2BK20ac were downregulated. Additionally, we found that CAT, SOD1 and SOD2 might be epigenetically regulated and involved in the pathogenesis of manganism. Conclusion: This study identified histone acetylation sites and oxidative stress-related genes associated with the pathogenesis of manganese toxicity, and these findings are useful in the search for potential epigenetic targets for manganese toxicity.

Tweetable abstract

We identified specific histone acetylation sites associated with manganese toxicity, which will help to explore targets for pharmacological intervention in manganese toxicity as well as pathogenesis studies.

Keywords::

• histone acetylation
• LC–MS/MS
• manganese
• oxidative stress
• proteomics

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.futuremedicine.com/doi/suppl/10.2217/epi-2023-0364

Author contributions

C Ao drafted the manuscript. S Tang, Y Yang, Y Liu and H Zhao contributed to the analysis and interpretation of the data. S Tang and Y Yang performed the experiments and collected the data. J Ban and J Li designed the study and revised the manuscript. All authors gave final approval of the version to be published and agree to take responsibility for all aspects of the work.

Acknowledgments

The authors are grateful to the School of Public Health, Guizhou Medical University and the Key Laboratory of Environmental Pollution Monitoring and Disease Control of the Ministry of Education for their support.

Financial disclosure

This work was supported by the National Natural Science Foundation of China (grant no. 81960578). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

Competing interests disclosure

The authors have no competing interests or relevant affiliations with any organization or entity with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

Writing disclosure

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained the Guizhou Medical University Laboratory Animal Care and Use Committee approval.

Additional information

Funding

This work was supported by the National Natural Science Foundation of China (grant no. 81960578). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.