Association of PON1 Gene Polymorphisms and Enzymatic Activity With Risk of Coronary Artery Disease

Abstract

Background: The present case–control study evaluated the association of PON1 gene polymorphisms and enzyme activity in the western Indian population. Materials & methods: Angiographically proven coronary artery disease (CAD) formed the cases. PON1 polymorphisms (Q192R, L55M) and enzymatic activity (paraoxonase) were assessed. Results: A total of 502 participants (251 per group) were studied. PON1 Q192R and L55M polymorphisms were not associated with the risk of CAD. Notably, a weak association was observed between Q192R polymorphisms and the risk of CAD. CAD patients had significantly lower PON1 enzymatic activity (U/L) as compared with the controls regardless of the genotype. Conclusion: Low serum PON1 activity was confirmed to be an independent predictor for the risk of CAD.

Keywords:

• atherosclerosis
• paraoxonase
• polymorphism

Author contributions

CJ Godbole was the main contributor in concept, design and conduct of the study. CJ Godbole analyzed and interpreted the study data. SJ Thaker was the main contributor in the design of the study and manuscript writing. SJ Thaker helped in the statistical analysis and interpretation of data. PG Kerkar was the main contributor in the recruitment of patients and helped in interpretation of the results and manuscript review. MY Nadkar helped in the recruitment of patients and manuscript review. NJ Gogtay helped in the statistical analysis and was the main contributor in manuscript review. UM Thatte helped in the design and conduct of the study, manuscript writing and manuscript review.

All the authors have given the final approval of the version to be published. All the authors agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Financial & competing interests disclosure

This work was supported by the Diamond Jubilee Society Trust (Junior Teacher grant) and Research Society of the Seth GS Medical College & KEM Hospital, Parel, Mumbai 400012. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.

The authors state that they have obtained verbal and written informed consent from the patient/patients for the inclusion of their medical and treatment history within this case report.

Additional information

Funding

This work was supported by the Diamond Jubilee Society Trust (Junior Teacher grant) and Research Society of the Seth GS Medical College & KEM Hospital, Parel, Mumbai 400012. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.