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Abstract
Evaluation of: Fan AC, Deb-Basu D, Orban MW et al.: Nanofluidic proteomic assay for serial analysis of oncoprotein activation in clinical specimens. Nat. Med. 15, 566–571 (2009). In order to detect oncoprotein expression and phosphorylation in a small amount of cells, the authors developed a nanofluidic proteomic immunoassay. Several different settings were tested. This method showed results in protein detection comparable with western blot in both in vitro and in vivo experiments. It was demonstrated that the nanofluidic proteomic immunoassay can be used for assessment of protein phosphorylation changes, and thus could be useful for therapy monitoring. Detection of protein isoforms was also carried out for in vitro and in vivo settings. In conclusion, a method that combines isoelectric focusing of proteins and antibody detection of specific targets with chemiluminescence is applicable in many preclinical trials, as well as for the monitoring of disease treatment in practice.
Financial & competing interests disclosure
The author has no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.
No writing assistance was utilized in the production of this manuscript.