Elevated Levels of Procoagulant Microvesicles in Patients With Dengue Fever

Abstract

Background: The levels of procoagulant microvesicles (MVs) and tissue factor (TF)-bearing MVs may be increased in many conditions, including dengue fever (DF). This study aimed to measure the levels of MVs and TF-bearing MVs in patients with DF and matched healthy controls. Materials & methods: Levels of MVs and TF-bearing MVs in the plasma of patients with DF and matched healthy controls were measured using functional assay. Results: The patient group had significantly elevated levels of MVs (p < 0.001) and slightly increased levels of TF-bearing MVs (p = 0.454) compared with the matched healthy controls. Conclusion: Elevated levels of MVs and TF-bearing MVs could be used as biomarkers to evaluate the hemostatic function of patients with DF.

Keywords:

• dengue hemorrhagic fever
• dengue infection
• dengue shock syndrome
• dengue virus
• microvesicles
• procoagulant
• tissue factor-bearing microvesicles

Author contributions

HA Hamali designed the study. HA Hamali, AA Mobarki and M Saboor also contributed toward the manuscript. G Dobie, AJ Halawani, ZM Eisa, Y Hobani and AM Hakami collected the samples, HA Hamali, MS Akhter, AM Madkhali, AA Mobarki performed the MVs analysis, HA Hamali, AA Mobarki, AM Madkhali, AJ Halawani and M Saboor reviewed and edited the manuscript.

Acknowledgments

The authors acknowledge the deanship of Scientific Research at Jazan University, Saudi Arabia.

Financial & competing interests disclosure

This work was supported by the 8th Scientific Research Program, Deanship of Scientific Research, Jazan University grant number JUP8/000355, Saudi Arabia. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

Enago editing and proofreading services were used.

Ethical conduct of research

The current study was approved by the Scientific Research Ethics Committee of Jazan University (reference number REC39/6-355). In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.